Detailed information of TA system TA164431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4921442..4922058
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2I8SCX0
• Locus tag: HV039_RS23730
• Protein ID: WP_049269424.1
• Coordinates: 4921442..4921816 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: HV039_RS23735
• Protein ID: WP_016155181.1
• Coordinates: 4921816..4922058 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS23715	4918945..4919847	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV039_RS23720	4919844..4920479	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV039_RS23725	4920476..4921405	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV039_RS23730	4921442..4921816	-	375	WP_049269424.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV039_RS23735	4921816..4922058	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV039_RS23740	4922264..4923172	+	909	WP_049269423.1	alpha/beta hydrolase	-
HV039_RS23745	4923237..4923479	+	243	WP_016155179.1	type II toxin-antitoxin system ParD family antitoxin	-
HV039_RS23750	4923472..4923759	+	288	WP_049269420.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV039_RS23755	4923765..4924706	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV039_RS23760	4924751..4925188	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV039_RS23765	4925185..4926057	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV039_RS23770	4926051..4926580	-	530	Protein_4648	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13650.78 Da
• Isoelectric Point: 8.5373

>T164431 WP_049269424.1 NZ_CP056896:c4921816-4921442 [Citrobacter sp. RHBSTW-00021]
MATGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T164431 NZ_CP072914:c4607595-4607338 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT164431 WP_016155181.1 NZ_CP056896:c4922058-4921816 [Citrobacter sp. RHBSTW-00021]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT164431 NZ_CP072914:c4607327-4606776 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACTCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCCGTTGGACATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCACTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8SCX0

Antitoxin

• AlphaFold DB: R8WIS4