Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4921442..4922058 Replicon chromosome
Accession NZ_CP056896
Organism Citrobacter sp. RHBSTW-00021

Toxin (Protein)


Gene name vapC Uniprot ID A0A2I8SCX0
Locus tag HV039_RS23730 Protein ID WP_049269424.1
Coordinates 4921442..4921816 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag HV039_RS23735 Protein ID WP_016155181.1
Coordinates 4921816..4922058 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV039_RS23715 4918945..4919847 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV039_RS23720 4919844..4920479 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV039_RS23725 4920476..4921405 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV039_RS23730 4921442..4921816 - 375 WP_049269424.1 type II toxin-antitoxin system VapC family toxin Toxin
HV039_RS23735 4921816..4922058 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein Antitoxin
HV039_RS23740 4922264..4923172 + 909 WP_049269423.1 alpha/beta hydrolase -
HV039_RS23745 4923237..4923479 + 243 WP_016155179.1 type II toxin-antitoxin system ParD family antitoxin -
HV039_RS23750 4923472..4923759 + 288 WP_049269420.1 type II toxin-antitoxin system RelE/ParE family toxin -
HV039_RS23755 4923765..4924706 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV039_RS23760 4924751..4925188 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV039_RS23765 4925185..4926057 - 873 WP_019077938.1 virulence factor BrkB family protein -
HV039_RS23770 4926051..4926580 - 530 Protein_4648 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13650.78 Da        Isoelectric Point: 8.5373

>T164431 WP_049269424.1 NZ_CP056896:c4921816-4921442 [Citrobacter sp. RHBSTW-00021]
MATGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

>T164431 NZ_CP072914:c4607595-4607338 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT164431 WP_016155181.1 NZ_CP056896:c4922058-4921816 [Citrobacter sp. RHBSTW-00021]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp

>AT164431 NZ_CP072914:c4607327-4606776 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACTCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCCGTTGGACATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCACTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8SCX0

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References