Detailed information of TA system TA164430

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4622338..4623091
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: HV039_RS22375
• Protein ID: WP_016154457.1
• Coordinates: 4622714..4623091 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV039_RS22370
• Protein ID: WP_162838704.1
• Coordinates: 4622338..4622664 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS22310	4617397..4617663	+	267	WP_032950091.1	hypothetical protein	-
HV039_RS22315	4617676..4617987	+	312	WP_009652410.1	hypothetical protein	-
HV039_RS22320	4618034..4618504	+	471	WP_009652431.1	hypothetical protein	-
HV039_RS22325	4618553..4619065	+	513	WP_032950094.1	DUF4234 domain-containing protein	-
HV039_RS22330	4619147..4619368	+	222	WP_032950095.1	hypothetical protein	-
HV039_RS22335	4619424..4619657	+	234	WP_009652474.1	DUF905 domain-containing protein	-
HV039_RS22340	4619761..4620579	+	819	WP_009652424.1	DUF945 domain-containing protein	-
HV039_RS22345	4620579..4620833	+	255	WP_009652519.1	hypothetical protein	-
HV039_RS22350	4620862..4621323	+	462	WP_045890390.1	antirestriction protein	-
HV039_RS22355	4621334..4621552	+	219	WP_009652503.1	hypothetical protein	-
HV039_RS22360	4621564..4622046	+	483	WP_016154455.1	DNA repair protein RadC	-
HV039_RS22365	4622060..4622281	+	222	WP_009652392.1	DUF987 domain-containing protein	-
HV039_RS22370	4622338..4622664	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV039_RS22375	4622714..4623091	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
HV039_RS22380	4623088..4623579	+	492	WP_009652438.1	hypothetical protein	-
HV039_RS22385	4623609..4623812	+	204	WP_009652505.1	DUF957 domain-containing protein	-
HV039_RS22390	4623893..4624738	+	846	Protein_4388	DUF4942 domain-containing protein	-
HV039_RS22395	4624750..4624986	+	237	WP_016154458.1	hypothetical protein	-
HV039_RS22400	4625250..4625510	+	261	WP_158655782.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T164430 WP_016154457.1 NZ_CP056896:4622714-4623091 [Citrobacter sp. RHBSTW-00021]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T164430 NZ_CP072914:4368372-4368590 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT164430 WP_162838704.1 NZ_CP056896:4622338-4622664 [Citrobacter sp. RHBSTW-00021]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT164430 NZ_CP072914:4367971-4368345 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: R8WN26