Detailed information of TA system TA164427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4026707..4027283
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A2I8S420
• Locus tag: HV039_RS19545
• Protein ID: WP_049269953.1
• Coordinates: 4026707..4026994 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A2I8S1U6
• Locus tag: HV039_RS19550
• Protein ID: WP_016155614.1
• Coordinates: 4026981..4027283 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS19510	4022015..4023079	+	1065	WP_049041982.1	DUF2955 domain-containing protein	-
HV039_RS19515	4023354..4023716	+	363	WP_046275905.1	DUF2778 domain-containing protein	-
HV039_RS19520	4023716..4024222	+	507	WP_047414535.1	hypothetical protein	-
HV039_RS19525	4024223..4024714	+	492	WP_016151575.1	type VI secretion system tube protein Hcp	-
HV039_RS19530	4024845..4025143	-	299	Protein_3830	type I addiction module toxin, SymE family	-
HV039_RS19535	4025106..4025246	+	141	WP_165500049.1	hypothetical protein	-
HV039_RS19540	4025309..4026475	-	1167	WP_019078303.1	restriction endonuclease	-
HV039_RS19545	4026707..4026994	+	288	WP_049269953.1	BrnT family toxin	Toxin
HV039_RS19550	4026981..4027283	+	303	WP_016155614.1	BrnA antitoxin family protein	Antitoxin
HV039_RS19555	4027591..4027869	-	279	Protein_3835	ATP-binding domain-containing protein	-
HV039_RS19560	4027940..4029060	+	1121	WP_103283453.1	IS3 family transposase	-
HV039_RS19565	4029089..4030663	-	1575	Protein_3837	AAA family ATPase	-
HV039_RS19570	4030850..4031806	-	957	WP_049040419.1	GTPase	-
HV039_RS19575	4031819..4032013	-	195	WP_181500139.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11433.87 Da
• Isoelectric Point: 8.6051

>T164427 WP_049269953.1 NZ_CP056896:4026707-4026994 [Citrobacter sp. RHBSTW-00021]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESRFEVIRIIS
ARKADRKERNRYEHS

Nucleotide

• Length: 288 bp

>T164427 NZ_CP072914:918850-919239 [Klebsiella oxytoca]
ATGCAAACCAAATCATTCCCTCTCATGCGGGCGGCATTGTCACGTCCGTCTCCCGTTGATGTCTGGCAGGCTCTGCTGAC
CAGCCTGCTGGAACACCATTATGGCCTCACGCTTAATGACACGCCGTTCAGTGATGAACAGGTGATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTCATTGTTGAAAAGTACGAACTGGTCCGCATTGACCGGCCGGGCTTC
AGCATTATGGAGCAGTCTCCGTTTATCACACCCATTGATATCCTCCGGGCCAGAAAGGCCACGGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACAACCGCAGGCCAATACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11466.12 Da
• Isoelectric Point: 10.1293

>AT164427 WP_016155614.1 NZ_CP056896:4026981-4027283 [Citrobacter sp. RHBSTW-00021]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT164427 NZ_CP072914:918468-918794 [Klebsiella oxytoca]
ATCAGCGAACCCTGGTGGGGGCTAAGACGCAGTGTCTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGAAATCGCCT
GCATTACCTGGCTGACCGGGCAAACTTTGATGGTCAGTTCTGTGATGCGGATTTGCGCCATCTTGACCAGTCTTTCCCGG
TACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACGGCGTCACACTGTATGCA
AAAGGTCTGGCCTGTGAGGCCGATACGCTCGGTTCGCATGGTTACGTTTATATCGCGATTTATCCCGCTCCCGTCACCAC
AGAATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S420

Antitoxin

• AlphaFold DB: A0A2I8S1U6