Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3885020..3885699 | Replicon | chromosome |
| Accession | NZ_CP056896 | ||
| Organism | Citrobacter sp. RHBSTW-00021 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | A0A2I8S2T2 |
| Locus tag | HV039_RS18860 | Protein ID | WP_008324514.1 |
| Coordinates | 3885358..3885699 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | A0A2I8S2U6 |
| Locus tag | HV039_RS18855 | Protein ID | WP_008324516.1 |
| Coordinates | 3885020..3885337 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HV039_RS18810 | 3880461..3881282 | + | 822 | WP_032654809.1 | DUF945 domain-containing protein | - |
| HV039_RS18815 | 3881500..3882201 | + | 702 | WP_008324530.1 | WYL domain-containing protein | - |
| HV039_RS18820 | 3882238..3882474 | + | 237 | WP_001115854.1 | hypothetical protein | - |
| HV039_RS18825 | 3882474..3882917 | + | 444 | WP_000824223.1 | hypothetical protein | - |
| HV039_RS18830 | 3882940..3883407 | + | 468 | WP_001547765.1 | hypothetical protein | - |
| HV039_RS18835 | 3883484..3883723 | + | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| HV039_RS18840 | 3883821..3884279 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| HV039_RS18845 | 3884295..3884771 | + | 477 | WP_000811693.1 | RadC family protein | - |
| HV039_RS18850 | 3884780..3885007 | + | 228 | WP_008324517.1 | DUF987 domain-containing protein | - |
| HV039_RS18855 | 3885020..3885337 | + | 318 | WP_008324516.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| HV039_RS18860 | 3885358..3885699 | + | 342 | WP_008324514.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| HV039_RS18870 | 3886319..3886783 | - | 465 | WP_103283511.1 | isoprenylcysteine carboxylmethyltransferase family protein | - |
| HV039_RS18875 | 3886977..3888425 | - | 1449 | WP_103283510.1 | EAL domain-containing protein | - |
| HV039_RS18880 | 3888768..3889241 | - | 474 | WP_016155774.1 | hypothetical protein | - |
| HV039_RS18885 | 3889216..3889947 | - | 732 | WP_049270882.1 | winged helix-turn-helix domain-containing protein | - |
| HV039_RS18890 | 3890092..3890451 | - | 360 | Protein_3703 | molecular chaperone | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3830525..3907952 | 77427 | |
| - | flank | IS/Tn | - | - | 3890461..3891612 | 1151 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12919.98 Da Isoelectric Point: 9.6543
>T164426 WP_008324514.1 NZ_CP056896:3885358-3885699 [Citrobacter sp. RHBSTW-00021]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T164426 NZ_CP072914:861710-862120 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACTCCGTGGGTAATCAATAGCGGAATGCTGCTGCGCCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACTCCGTGGGTAATCAATAGCGGAATGCTGCTGCGCCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11787.33 Da Isoelectric Point: 7.3476
>AT164426 WP_008324516.1 NZ_CP056896:3885020-3885337 [Citrobacter sp. RHBSTW-00021]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS
Download Length: 318 bp
>AT164426 NZ_CP072914:861463-861729 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2I8S2T2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2I8S2U6 |