Detailed information of TA system TA164425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3675437..3676057
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV039_RS17880
• Protein ID: WP_002892050.1
• Coordinates: 3675839..3676057 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV039_RS17875
• Protein ID: WP_181500127.1
• Coordinates: 3675437..3675811 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS17865	3670584..3671777	+	1194	WP_016152074.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV039_RS17870	3671800..3674949	+	3150	WP_016152073.1	multidrug efflux RND transporter permease subunit	-
HV039_RS17875	3675437..3675811	+	375	WP_181500127.1	Hha toxicity modulator TomB	Antitoxin
HV039_RS17880	3675839..3676057	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV039_RS17885	3676238..3676789	+	552	WP_103283555.1	maltose O-acetyltransferase	-
HV039_RS17890	3676906..3677376	+	471	WP_016152071.1	YlaC family protein	-
HV039_RS17895	3677454..3677594	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV039_RS17900	3677596..3677856	-	261	WP_016152070.1	type B 50S ribosomal protein L31	-
HV039_RS17905	3678045..3679598	+	1554	WP_047416867.1	EAL domain-containing protein	-
HV039_RS17910	3679650..3680003	-	354	WP_003831033.1	DUF1428 family protein	-
HV039_RS17915	3680068..3680697	-	630	WP_103283554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T164425 WP_002892050.1 NZ_CP056896:3675839-3676057 [Citrobacter sp. RHBSTW-00021]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T164425 NZ_CP072914:641893-642267 [Klebsiella oxytoca]
ATGAAATGGGTGAGCGCAAGCGAAGTCATTGCCTTTCATGATCGCATTTTACAACATCTCCCCGGCGTTAAAGGTATGTC
CGATCCTGGTCGGGCGGAAGCCATAATTTATCGTGTACAGAACCGCTTTCACTATGAAGGGGTGAACGACATTTTTGAAC
TGGCTGCAACCTATTGGGTCGCTATCGCGCGTGGGCACATTTTTAATGATGGTAATAAGAGGACTGCTTTTTTTATCACC
ATGACGTTCCTGGCCCGCAATGGGTATCTTATCGCTGATGAAGATACCCGGTTGGAAGAACTCACTGTGCTGGCTGCGAC
AGGCGAAGCTACGGTAGTAGTTCTTGCTGATGCGTTACGGCAATTGGCGCTCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14445.26 Da
• Isoelectric Point: 5.5653

>AT164425 WP_181500127.1 NZ_CP056896:3675437-3675811 [Citrobacter sp. RHBSTW-00021]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKMIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT164425 NZ_CP072914:641675-641896 [Klebsiella oxytoca]
ATGCGCTCTTATAGTACATCTGAAGCCAGACAGAATATTGCGGCCGTCATGGACGCCGCTGCCAGCGGAGAACCGATTGA
AATTACGCGGCGTGATGGAACGTCTAATGTTTTGATCAGTAAGGCTGAATATGAAACGTTTATGAATGCCAAACTTGATG
CGGAGTTTGATTTTATTATGCAGCGACACGGCCGTACAGTTAAGGCGTTGACCGATCGATGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2