Detailed information of TA system TA164415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2470500..2471136
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV039_RS11950
• Protein ID: WP_049259794.1
• Coordinates: 2470500..2470688 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV039_RS11955
• Protein ID: WP_146053235.1
• Coordinates: 2470720..2471136 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS11930	2467289..2467519	-	231	WP_016156585.1	DUF2554 family protein	-
HV039_RS11935	2467709..2467834	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV039_RS11940	2467834..2468844	-	1011	WP_103283796.1	cytochrome d ubiquinol oxidase subunit II	-
HV039_RS11945	2468844..2470236	-	1393	Protein_2339	cytochrome ubiquinol oxidase subunit I	-
HV039_RS11950	2470500..2470688	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV039_RS11955	2470720..2471136	+	417	WP_146053235.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV039_RS11960	2471229..2472638	+	1410	WP_103283798.1	PLP-dependent aminotransferase family protein	-
HV039_RS11965	2472968..2474113	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV039_RS11970	2474130..2475146	+	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
HV039_RS11975	2475147..2476091	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T164415 WP_049259794.1 NZ_CP056896:2470500-2470688 [Citrobacter sp. RHBSTW-00021]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T164415 NZ_CP072906:46437-46724 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.33 Da
• Isoelectric Point: 4.7119

>AT164415 WP_146053235.1 NZ_CP056896:2470720-2471136 [Citrobacter sp. RHBSTW-00021]
MRYPVNLIPSVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT164415 NZ_CP072906:46198-46437 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9