Detailed information of TA system TA164414

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 996658..997430
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ICB7
• Locus tag: HV039_RS04930
• Protein ID: WP_049238634.1
• Coordinates: 997041..997430 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV039_RS04925
• Protein ID: WP_171547612.1
• Coordinates: 996658..996984 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS04895	993004..993636	-	633	WP_049238458.1	inovirus Gp2 family protein	-
HV039_RS04900	994273..995097	+	825	WP_049238647.1	DUF945 domain-containing protein	-
HV039_RS04905	995094..995351	+	258	WP_049238645.1	DUF905 domain-containing protein	-
HV039_RS04910	995431..995874	+	444	WP_049238643.1	antirestriction protein	-
HV039_RS04915	995886..996365	+	480	WP_049238640.1	DNA repair protein RadC	-
HV039_RS04920	996379..996600	+	222	WP_049238638.1	DUF987 domain-containing protein	-
HV039_RS04925	996658..996984	+	327	WP_171547612.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV039_RS04930	997041..997430	+	390	WP_049238634.1	TA system toxin CbtA family protein	Toxin
HV039_RS04935	997517..998356	+	840	WP_049238632.1	DUF4942 domain-containing protein	-
HV039_RS04940	999771..1000298	+	528	WP_019077624.1	hypothetical protein	-
HV039_RS04945	1000556..1001833	+	1278	WP_016157409.1	Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tviB / tviC	961388..1002876	41488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14387.45 Da
• Isoelectric Point: 9.6254

>T164414 WP_049238634.1 NZ_CP056896:997041-997430 [Citrobacter sp. RHBSTW-00021]
MQTKLSPLMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIDAGISLSDALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKAAGLMNRGTYKEVTAITRGQHPQANASGKR

Nucleotide

• Length: 390 bp

>T164414 NZ_CP072906:37840-38310 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTATCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGAATCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12174.84 Da
• Isoelectric Point: 6.4673

>AT164414 WP_171547612.1 NZ_CP056896:996658-996984 [Citrobacter sp. RHBSTW-00021]
ISEPWWGLRRSVSPCFGARLVQERNSLHYLADRANFDGQFCDADLRYLDQAFPVLMKQLELMLTSGELNPRHQHGVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVTTA

Nucleotide

• Length: 327 bp

>AT164414 NZ_CP072906:37400-37843 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7L6ICB7