Detailed information of TA system TA164413

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 950604..951258
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV039_RS04645
• Protein ID: WP_016154348.1
• Coordinates: 950851..951258 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV039_RS04640
• Protein ID: WP_016154349.1
• Coordinates: 950604..950870 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS04615	945817..947250	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV039_RS04620	947371..948099	-	729	WP_103284472.1	MurR/RpiR family transcriptional regulator	-
HV039_RS04625	948152..948463	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV039_RS04630	948627..949286	+	660	WP_016154351.1	hemolysin III family protein	-
HV039_RS04635	949366..950346	-	981	WP_016154350.1	tRNA-modifying protein YgfZ	-
HV039_RS04640	950604..950870	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV039_RS04645	950851..951258	+	408	WP_016154348.1	protein YgfX	Toxin
HV039_RS04650	951359..951880	-	522	WP_016154347.1	flavodoxin FldB	-
HV039_RS04655	951994..952890	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV039_RS04660	952914..953627	+	714	WP_075847456.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV039_RS04665	953633..955366	+	1734	WP_103284192.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T164413 WP_016154348.1 NZ_CP056896:950851-951258 [Citrobacter sp. RHBSTW-00021]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T164413 NZ_CP072905:4900335-4900817 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT164413 WP_016154349.1 NZ_CP056896:950604-950870 [Citrobacter sp. RHBSTW-00021]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164413 NZ_CP072905:4900081-4900347 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6