Detailed information of TA system TA164412

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 716339..717092
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV039_RS03440
• Protein ID: WP_181631211.1
• Coordinates: 716715..717092 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7T0E0C6
• Locus tag: HV039_RS03435
• Protein ID: WP_072269511.1
• Coordinates: 716339..716665 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS03415	711747..714902	+	3156	WP_181631210.1	AIDA repeat-containing protein	-
HV039_RS03420	715125..715556	+	432	WP_088903392.1	antirestriction protein	-
HV039_RS03425	715568..716047	+	480	WP_025798999.1	DNA repair protein RadC	-
HV039_RS03430	716061..716282	+	222	WP_045888217.1	DUF987 domain-containing protein	-
HV039_RS03435	716339..716665	+	327	WP_072269511.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV039_RS03440	716715..717092	+	378	WP_181631211.1	TA system toxin CbtA family protein	Toxin
HV039_RS03445	717089..717580	+	492	WP_045888216.1	hypothetical protein	-
HV039_RS03450	717609..717812	+	204	WP_025799007.1	DUF957 domain-containing protein	-
HV039_RS03455	717894..718739	+	846	WP_025799009.1	DUF4942 domain-containing protein	-
HV039_RS03465	719043..719549	+	507	WP_103284247.1	G/U mismatch-specific DNA glycosylase	-
HV039_RS03470	719595..721439	-	1845	WP_016154549.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13963.99 Da
• Isoelectric Point: 7.2909

>T164412 WP_181631211.1 NZ_CP056896:716715-717092 [Citrobacter sp. RHBSTW-00021]
MQTQPVPPTQEASPRPSPVEIWLRLLSHLLDRHYGLTLNDTPFCNDGVIQEHIDAGISLCDAVNFIVEKYALIRTDRHGF
SAETQSPLISSIDILRARKACGLMARNGYRLVTDITTGKYSVVTR

Nucleotide

• Length: 378 bp

>T164412 NZ_CP072905:4883617-4884033 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12016.71 Da
• Isoelectric Point: 6.4674

>AT164412 WP_072269511.1 NZ_CP056896:716339-716665 [Citrobacter sp. RHBSTW-00021]
IAEPWWGLKRDITPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELNPRHQHGVTLYA
KGLTCNADTLGSCGYVYIAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT164412 NZ_CP072905:4883390-4883620 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7T0E0C6