Detailed information of TA system TA164393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1785892..1786482
• Replicon: chromosome
• Accession: NZ_CP056883
• Organism: Klebsiella pneumoniae strain RHBSTW-00062

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV049_RS08755
• Protein ID: WP_032421545.1
• Coordinates: 1786150..1786482 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HV049_RS08750
• Protein ID: WP_096106567.1
• Coordinates: 1785892..1786149 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV049_RS08725	1781727..1781969	-	243	WP_022615591.1	hypothetical protein	-
HV049_RS08730	1782236..1783984	+	1749	WP_096106564.1	hypothetical protein	-
HV049_RS08735	1784063..1784611	+	549	WP_096106565.1	hypothetical protein	-
HV049_RS08740	1784894..1785354	+	461	Protein_1710	hypothetical protein	-
HV049_RS08745	1785351..1785557	+	207	WP_096106566.1	helix-turn-helix domain-containing protein	-
HV049_RS08750	1785892..1786149	+	258	WP_096106567.1	antitoxin	Antitoxin
HV049_RS08755	1786150..1786482	+	333	WP_032421545.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV049_RS08765	1786804..1788240	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HV049_RS08775	1788606..1790060	-	1455	WP_023286675.1	AMP nucleosidase	-
HV049_RS08780	1790190..1790435	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11917.79 Da
• Isoelectric Point: 10.0968

>T164393 WP_032421545.1 NZ_CP056883:1786150-1786482 [Klebsiella pneumoniae]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T164393 NZ_CP072904:c266271-266026 [Bartonella henselae]
TTGGCTTGGACGATTAGATATGAAAAAAAAGCTCTCAGTTTTTTAAAAAAATGCGATAAAAAAGAAGCACGGCGGATTGT
TGATTTTTTAGATCAACACGTTGCTCCTCTTGAAGATGTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCCCAGGTT
TATGGAGATATCGTGTAGGAGATTACAGGATACTTTGTGAACTCGTGGGAGTGGAGATATTGTTTTTAGAGCTCCAGGGA
TTATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9189.65 Da
• Isoelectric Point: 4.7468

>AT164393 WP_096106567.1 NZ_CP056883:1785892-1786149 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLGLSTDSEVGMTIDNGCLVIEPQKRPHYSLEELLAQCDPHAEISEGGREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT164393 NZ_CP072904:c266482-266255 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG