Detailed information of TA system TA164369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 141059..141585
• Replicon: plasmid pRHBSTW-00113_2
• Accession: NZ_CP056855
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HV075_RS25780
• Protein ID: WP_000323025.1
• Coordinates: 141059..141346 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: HV075_RS25785
• Protein ID: WP_004196370.1
• Coordinates: 141346..141585 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS25750	136257..136529	-	273	WP_032425552.1	hypothetical protein	-
HV075_RS25755	137039..138628	-	1590	WP_015632444.1	IS66 family transposase	-
HV075_RS25760	138658..139008	-	351	WP_032414478.1	IS66 family insertion sequence element accessory protein TnpB	-
HV075_RS25765	139005..139412	-	408	WP_015632445.1	transposase	-
HV075_RS25770	139728..139886	-	159	WP_014343509.1	type I toxin-antitoxin system Hok family toxin	-
HV075_RS25775	139958..140992	+	1035	Protein_156	IS481-like element ISKpn28 family transposase	-
HV075_RS25780	141059..141346	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HV075_RS25785	141346..141585	-	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HV075_RS25790	141836..142201	+	366	WP_009651956.1	hypothetical protein	-
HV075_RS25795	142245..142982	+	738	WP_008460272.1	HupE/UreJ family protein	-
HV075_RS25800	142996..143685	+	690	WP_004196322.1	hypothetical protein	-
HV075_RS25805	143716..145092	-	1377	Protein_162	chromate efflux transporter	-
HV075_RS25810	145049..146026	-	978	WP_004196334.1	chromate resistance protein	-
HV075_RS25815	146056..146248	+	193	Protein_164	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..188591	188591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T164369 WP_000323025.1 NZ_CP056855:c141346-141059 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T164369 NZ_CP072901:c1599801-1599601 [Bartonella henselae]
ATGGAACAAAATAGCCGAAAGATAATTGCAAAATTAAAACGCGATGGCTTTGAACTTGTCAAAGTAAAAGGTTCGCACCA
TAAATTTAAAAAAGATGGTAAGGTTGTTATTGTCCCACATCCTAAAAAAAATCTTCCAATTGGTACAGCGCGTTCTATTG
CACAACAAGCAGGCTGGTTTAAAAAAGGAGAAGAAGAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT164369 WP_004196370.1 NZ_CP056855:c141585-141346 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT164369 NZ_CP072901:c1599604-1599209 [Bartonella henselae]
ATGAAAAGATTTTTTGCTCTTGTTCATAAAGATGAGGATTCTGCTTTTGGTGTTCAATTTCCTGATTTTGAAGGTCTATT
CTCTGCTGCTGATGAAGAAGAAAATCTTATCATAAATGCAACCGAAGCTTTGCAACTTTACTGTGAAGATATGGATACAG
TGCCTGTTCCTTTAAAATTTGAAGAAGTGATACAACAGAAAGCTGTCAAAAAAGCTTTGTCAGAAGGAGCTTTTTTAATA
CAAGTTCCTTTTATTGAAAATGATTCAGAAGTCGTACGTACAAATATATCAATTGAACGAGGGCTTTTGCGTGCAATTGA
TAATTGTGCACAAGAGAGAGGGTTAACAAGATCTGCTTTTTTGGCAACAGCAGCCCGTCACGAGCTTAATATTTAG

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4