Detailed information of TA system TA164368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 56457..57193
• Replicon: plasmid pRHBSTW-00113_2
• Accession: NZ_CP056855
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: HV075_RS25310
• Protein ID: WP_004098919.1
• Coordinates: 56711..57193 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A455TK27
• Locus tag: HV075_RS25305
• Protein ID: WP_020804426.1
• Coordinates: 56457..56723 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS25260	52639..53073	+	435	WP_004118347.1	hypothetical protein	-
HV075_RS25265	53452..53550	-	99	WP_004118688.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV075_RS25270	53537..53716	-	180	WP_004118349.1	type II toxin-antitoxin system ParD family antitoxin	-
HV075_RS25275	54030..54293	+	264	WP_004118691.1	hypothetical protein	-
HV075_RS25280	54290..54856	+	567	WP_042922197.1	hypothetical protein	-
HV075_RS25285	54887..55381	+	495	WP_016528789.1	hypothetical protein	-
HV075_RS25290	55442..55645	+	204	WP_004150739.1	hemolysin expression modulator Hha	-
HV075_RS25295	55694..55951	+	258	WP_004098928.1	hypothetical protein	-
HV075_RS25300	56027..56281	+	255	WP_032425571.1	hypothetical protein	-
HV075_RS25305	56457..56723	+	267	WP_020804426.1	DUF1778 domain-containing protein	Antitoxin
HV075_RS25310	56711..57193	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
HV075_RS25315	57394..58797	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HV075_RS25320	58826..59170	-	345	WP_015056386.1	hypothetical protein	-
HV075_RS25325	59259..60227	+	969	WP_074185243.1	IS5 family transposase	-
HV075_RS25330	60742..61263	+	522	WP_004197062.1	RNA polymerase sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..188591	188591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T164368 WP_004098919.1 NZ_CP056855:56711-57193 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T164368 NZ_CP072901:1593875-1594156 [Bartonella henselae]
ATGCGGACGATTGAACGTACGACTATCTTCAAACGTGATTTCAAGCGTGAGATGAAAGGACAACATCGGCATCTTTTAGA
TACTGATTTACGCAAAGTCATCGCAGCATTAGCAAACGATCAACCTTTAGAGATACGGTACCGTGATCATGCATTGACTG
GAAATTGGAGTAATTACCGAGATTGTCATATTCGACCTGATCTAGTGTTGATTTATCGCTTGATTGGTCAAGATAGGCTA
GTTTTAGTGCGTCTTGGTTCTCATTCACAACTTGATCTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10124.47 Da
• Isoelectric Point: 4.3108

>AT164368 WP_020804426.1 NZ_CP056855:56457-56723 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT164368 NZ_CP072901:1593625-1593888 [Bartonella henselae]
ATGGCTACCAGTCGCATGGTTCAAGCACGTGTACCGGAAGAAATTCAAAATGTCGCTAGTCAAGTTATTCAAGCTTCAGG
TTTGACAGTGAGTGATGTTGTGAGGGTATTGATGACTCGTATCGCGCAAGATAAAGCTATTCCATCTGTTTTGTTTCAAC
CCAATGCAGAAACCATAGCAGCTTTTGCTGAAGTTGATGAAGGCAACTTAAAAAAGTTTCATTCCGTAGACGAATTATTT
GATGATCTTTATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A455TK27