Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25339..25982 Replicon plasmid pRHBSTW-00113_2
Accession NZ_CP056855
Organism Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag HV075_RS25140 Protein ID WP_020804312.1
Coordinates 25566..25982 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HV075_RS25135 Protein ID WP_001261276.1
Coordinates 25339..25569 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV075_RS25100 21121..21378 + 258 WP_009310077.1 hypothetical protein -
HV075_RS25105 21436..22215 - 780 WP_023287113.1 site-specific integrase -
HV075_RS25110 22413..23429 - 1017 WP_009309921.1 hypothetical protein -
HV075_RS25115 23463..23798 - 336 WP_009309920.1 hypothetical protein -
HV075_RS25120 23848..23988 - 141 WP_162898808.1 hypothetical protein -
HV075_RS25125 24217..24522 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
HV075_RS25130 24524..24742 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
HV075_RS25135 25339..25569 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV075_RS25140 25566..25982 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
HV075_RS25145 26023..26901 - 879 WP_009309916.1 restriction endonuclease -
HV075_RS25150 27563..28624 + 1062 Protein_31 HlyD family secretion protein -
HV075_RS25155 28773..29753 - 981 WP_009310076.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..188591 188591
- flank IS/Tn - - 20014..20994 980
- flank IS/Tn - - 28773..29753 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T164367 WP_020804312.1 NZ_CP056855:25566-25982 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T164367 NZ_CP072901:1592588-1592902 [Bartonella henselae]
ATGATACATAAAAGAACGAATGAAGGTGACTTGGTAATTGAATCTTTTGCGGATAAGCGGTGTAAAGATCTTTTAGAAGG
CAATCCACCTAAAGGTTTTCCCACAACTCTCGTGCGTATAGTCCAACGAAAATTATTTATGCTGGATAAAGCAGTTGATC
TCAAAGATTTACGCAGTCCTCCGGGTAATCGTTTAGAGGCATTGAAAGGAGAGCGTAAAGGTCAATATTCTATTCGTATT
AATGACCAGTTTCGCATTTGTTTTGAGTGGCGTTCTAATGGTGCCTATGAAGTTGAAATCGTCGATTATCATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT164367 WP_001261276.1 NZ_CP056855:25339-25569 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT164367 NZ_CP072901:1592919-1593215 [Bartonella henselae]
ATGAAAAATTATATTGCTATTCATCCCGGAGAAATTTTACGGGAAGAATATTTAAAAGAATATGCTCTCTCTGCTTATGC
TCTTGCCAAAGCCTTGAATGTTCCACGCACTCGGATAGAACGGATTGTAGCAGAAAATAGTCGGATAACTCCTGATACAG
CACTACGATTAGCCTCTTTTTTTGATACAACAGCTGAATTCTGGCTCAACATGCAAGCTGCTTACGATGTCAGTGTCTTA
CAAGCTGAAAAAGCAGATGAATTTTCCAAAATTAGTAAATTTGAATGTAGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References