Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 24217..24742 Replicon plasmid pRHBSTW-00113_2
Accession NZ_CP056855
Organism Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag HV075_RS25125 Protein ID WP_004197633.1
Coordinates 24217..24522 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV075_RS25130 Protein ID WP_006788213.1
Coordinates 24524..24742 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV075_RS25095 20014..20994 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
HV075_RS25100 21121..21378 + 258 WP_009310077.1 hypothetical protein -
HV075_RS25105 21436..22215 - 780 WP_023287113.1 site-specific integrase -
HV075_RS25110 22413..23429 - 1017 WP_009309921.1 hypothetical protein -
HV075_RS25115 23463..23798 - 336 WP_009309920.1 hypothetical protein -
HV075_RS25120 23848..23988 - 141 WP_162898808.1 hypothetical protein -
HV075_RS25125 24217..24522 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
HV075_RS25130 24524..24742 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV075_RS25135 25339..25569 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV075_RS25140 25566..25982 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
HV075_RS25145 26023..26901 - 879 WP_009309916.1 restriction endonuclease -
HV075_RS25150 27563..28624 + 1062 Protein_31 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..188591 188591
- flank IS/Tn - - 20014..20994 980
- flank IS/Tn - - 28773..29753 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T164366 WP_004197633.1 NZ_CP056855:c24522-24217 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T164366 NZ_CP072901:c296421-296269 [Bartonella henselae]
GTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCTCAGGTTTATGGAGATATCGTGTAGGAGATTACAGGATACTTTG
TGAACTCTATGATAAGGAGCTTGTCGTATTAGTTTTGGCTGTTGGACATAGAAAAAATATATATAAAGGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT164366 WP_006788213.1 NZ_CP056855:c24742-24524 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT164366 NZ_CP072901:c296748-296521 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAAGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References