Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5046446..5047071 Replicon chromosome
Accession NZ_CP056854
Organism Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV075_RS24610 Protein ID WP_002882817.1
Coordinates 5046446..5046829 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV075_RS24615 Protein ID WP_004150355.1
Coordinates 5046829..5047071 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV075_RS24595 5043812..5044714 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV075_RS24600 5044711..5045346 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV075_RS24605 5045343..5046272 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV075_RS24610 5046446..5046829 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV075_RS24615 5046829..5047071 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV075_RS24620 5047276..5048193 + 918 WP_004181612.1 alpha/beta hydrolase -
HV075_RS24625 5048207..5049148 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV075_RS24630 5049193..5049630 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV075_RS24635 5049627..5050487 - 861 WP_023280688.1 virulence factor BrkB family protein -
HV075_RS24640 5050481..5051080 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T164365 WP_002882817.1 NZ_CP056854:c5046829-5046446 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T164365 NZ_CP072901:264583-264855 [Bartonella henselae]
ATGAAGCTCGTTTGGACACGGATAGCGCATTACGATCGTCAAAAGATACGGGAATATATCGCGCAAGAAAATCCTTCTGC
TGCATTAGTATTCGACAAGCTTTTATCTAAAAAAGTAGAAAGACTCATTAGCTTTCCTAATCTTGGTCGTGTTGGCCGAA
TTAGGAATACGCGCGAGCTTATTGTGCGTAAGAATTATATTATGATTTATGATGTTTCTAATGGCACTATACGGGTTTTG
CGCATACTTCACGCAAAACAAAAGTGGCCATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT164365 WP_004150355.1 NZ_CP056854:c5047071-5046829 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT164365 NZ_CP072901:264296-264586 [Bartonella henselae]
ATGGCTGAAACAACATTTACCTTTCGCGTTGATGATGCATTAAAAAGTGAATTTTCTCAGGCTGCAAAAGCATGTGATCG
ATCTGCTGCACAGCTCTTACGGGATTACATGCGTGATATTGTAAAAGAACAAAAAGAAAAGATAGCGCATGAATTATGGT
TTCAGGAACAAGTCCAACTTGGATTAAATTCTGCCAACGCAGGTGATGTAATACCTTTTGAAGAAATTGAAACAGAAGCA
CAAGCATGGCGCTTTGAAATACAACGCAAGCTGAAGACATCAGATTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References