Detailed information of TA system TA164361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3888382..3889001
• Replicon: chromosome
• Accession: NZ_CP056854
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV075_RS19105
• Protein ID: WP_002892050.1
• Coordinates: 3888783..3889001 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV075_RS19100
• Protein ID: WP_181586270.1
• Coordinates: 3888382..3888756 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS19090	3883534..3884727	+	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV075_RS19095	3884750..3887896	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
HV075_RS19100	3888382..3888756	+	375	WP_181586270.1	Hha toxicity modulator TomB	Antitoxin
HV075_RS19105	3888783..3889001	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV075_RS19110	3889164..3889730	+	567	WP_002892030.1	maltose O-acetyltransferase	-
HV075_RS19115	3889702..3889842	-	141	WP_004147370.1	hypothetical protein	-
HV075_RS19120	3889863..3890333	+	471	WP_002892026.1	YlaC family protein	-
HV075_RS19125	3890308..3891759	-	1452	WP_021313732.1	PLP-dependent aminotransferase family protein	-
HV075_RS19130	3891860..3892558	+	699	WP_002892021.1	GNAT family N-acetyltransferase	-
HV075_RS19135	3892555..3892695	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
HV075_RS19140	3892695..3892958	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T164361 WP_002892050.1 NZ_CP056854:3888783-3889001 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T164361 NZ_CP072900:c716663-716349 [Bartonella henselae]
ATGATACATAAAAGAACGAATGAAGGTGACTTGGTAATTGAATCTTTTGCGGATAAGCGGTGTAAAGATCTTTTAGAAGG
CAATCCACCTAAAGGTTTTCCCACAACTCTCGTGCGTATAGTCCAACGAAAATTATTTATGCTGGATAAAGCAGTTGATC
TCAAAGATTTACGCAGTCCTCCGGGTAATCGTTTAGAGGCATTGAAAGGAGAGCGTAAAGGTCAATATTCTATTCGTATT
AATGACCAGTTTCGCATTTGTTTTGAGTGGCGTTCTAATGGTGCCTATGAAGTTGAAATCGTCGATTATCATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14396.07 Da
• Isoelectric Point: 4.8989

>AT164361 WP_181586270.1 NZ_CP056854:3888382-3888756 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNRLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT164361 NZ_CP072900:c716332-716036 [Bartonella henselae]
ATGAAAAATTATATTGCTATTCATCCCGGAGAAATTTTACGGGAAGAATATTTAAAAGAATATGCTCTCTCTGCTTATGC
TCTTGCCAAAGCCTTGAATGTTCCACGCACTCGGATAGAACGGATTGTAGCAGAAAATAGTCGGATAACTCCTGATACAG
CACTACGATTAGCCTCTTTTTTTGATACAACAGCTGAATTCTGGCTCAACATGCAAGCTGCTTACGATGTCAGTGTCTTA
CAAGCTGAAAAAGCAGATGAATTTTCCAAAATTAGTAAATTTGAATGTAGAGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2