Detailed information of TA system TA164350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 731991..732769
• Replicon: chromosome
• Accession: NZ_CP056854
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV075_RS03650
• Protein ID: WP_031591140.1
• Coordinates: 732281..732769 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: HV075_RS03645
• Protein ID: WP_004150912.1
• Coordinates: 731991..732284 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS03625	727199..727801	-	603	WP_004174410.1	short chain dehydrogenase	-
HV075_RS03630	727899..728810	+	912	WP_004181308.1	LysR family transcriptional regulator	-
HV075_RS03635	728811..729959	-	1149	WP_004174416.1	PLP-dependent aspartate aminotransferase family protein	-
HV075_RS03640	729970..731346	-	1377	WP_004217775.1	cystathionine beta-synthase	-
HV075_RS03645	731991..732284	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
HV075_RS03650	732281..732769	+	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
HV075_RS03655	732850..736593	-	3744	Protein_716	hypothetical protein	-
HV075_RS03660	736636..737664	-	1029	Protein_717	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T164350 WP_031591140.1 NZ_CP056854:732281-732769 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T164350 NZ_CP072899:c331029-330877 [Bartonella henselae]
GTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCTCAGGTTTATGGAGATATCGTGTAGGAGATTACAGGATACTTTG
TGAACTCTATGATAAGGAGCTTGTCGTATTAGTTTTGGCTGTTGGACATAGAAAAAATATATATAAAGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT164350 WP_004150912.1 NZ_CP056854:731991-732284 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT164350 NZ_CP072899:c331356-331129 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAAGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4