Detailed information of TA system TA164349

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 659601..660355
• Replicon: chromosome
• Accession: NZ_CP056854
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: HV075_RS03265
• Protein ID: WP_032669201.1
• Coordinates: 659978..660355 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV075_RS03260
• Protein ID: WP_074143921.1
• Coordinates: 659601..659927 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS03215	655403..655750	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
HV075_RS03220	655747..656151	-	405	WP_023290559.1	IS66 family insertion sequence hypothetical protein	-
HV075_RS03225	656234..656668	+	435	WP_177713015.1	hypothetical protein	-
HV075_RS03230	656665..657114	+	450	WP_032669197.1	hypothetical protein	-
HV075_RS03235	657186..657417	+	232	Protein_633	DUF905 domain-containing protein	-
HV075_RS03240	657516..658337	+	822	WP_032669198.1	DUF945 domain-containing protein	-
HV075_RS03245	658368..658811	+	444	WP_032669199.1	antirestriction protein	-
HV075_RS03250	658823..659302	+	480	WP_032669200.1	DNA repair protein RadC	-
HV075_RS03255	659323..659544	+	222	WP_023567997.1	DUF987 domain-containing protein	-
HV075_RS03260	659601..659927	+	327	WP_074143921.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV075_RS03265	659978..660355	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
HV075_RS03270	660352..660843	+	492	WP_032672023.1	hypothetical protein	-
HV075_RS03275	660873..661076	+	204	WP_032669203.1	DUF957 domain-containing protein	-
HV075_RS03280	661140..661988	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
HV075_RS03290	662291..662797	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
HV075_RS03295	662896..664737	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T164349 WP_032669201.1 NZ_CP056854:659978-660355 [Klebsiella pneumoniae]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T164349 NZ_CP072899:299182-299454 [Bartonella henselae]
ATGAAGCTCGTTTGGACACGGATAGCGCATTACGATCGTCAAAAGATACGGGAATATATCGCGCAAGAAAATCCTTCTGC
TGCATTAGTATTCGACAAGCTTTTATCTAAAAAAGTAGAAAGACTCATTAGCTTTCCTAATCTTGGTCGTGTTGGCCGAA
TTAGGAATACGCGCGAGCTTATTGTGCGTAAGAATTATATTATGATTTATGATGTTTCTAATGGCACTATACGGGTTTTG
CGCATACTTCACGCAAAACAAAAGTGGCCATAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11953.71 Da
• Isoelectric Point: 6.8397

>AT164349 WP_074143921.1 NZ_CP056854:659601-659927 [Klebsiella pneumoniae]
ITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELNPCHQHCVTLYA
KGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT164349 NZ_CP072899:298895-299185 [Bartonella henselae]
ATGGCTGAAACAACATTTACCTTTCGCGTTGATGATGCATTAAAAAGTGAATTTTCTCAGGCTGCAAAAGCATGTGATCG
ATCTGCTGCACAGCTCTTACGGGATTACATGCGTGATATTGTAAAAGAACAAAAAGAAAAGATAGCGCATGAATTATGGT
TTCAGGAACAAGTCCAACTTGGATTAAATTCTGCCAACGCAGGTGATGTAATACCTTTTGAAGAAATTGAAACAGAAGCA
CAAGCATGGCGCTTTGAAATACAACGCAAGCTGAAGACATCAGATTCATGA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6