Detailed information of TA system TA164348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 331560..332146
• Replicon: chromosome
• Accession: NZ_CP056854
• Organism: Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HV075_RS01555
• Protein ID: WP_135727996.1
• Coordinates: 331778..332146 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: HV075_RS01550
• Protein ID: WP_004174006.1
• Coordinates: 331560..331781 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV075_RS01530	327717..328643	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV075_RS01535	328640..329917	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
HV075_RS01540	329914..330681	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV075_RS01545	330683..331396	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV075_RS01550	331560..331781	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV075_RS01555	331778..332146	+	369	WP_135727996.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV075_RS01560	332419..333735	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV075_RS01565	333842..334729	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV075_RS01570	334726..335571	+	846	WP_004185988.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV075_RS01575	335573..336643	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	321328..337380	16052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13558.93 Da
• Isoelectric Point: 8.6410

>T164348 WP_135727996.1 NZ_CP056854:331778-332146 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTSGVAGMPDLGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T164348 NZ_CP072899:c297351-297019 [Bartonella henselae]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT164348 WP_004174006.1 NZ_CP056854:331560-331781 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT164348 NZ_CP072899:c297608-297351 [Bartonella henselae]
ATGCACACAACAAAATTACGTAAAGTTGGAGGGTCTGTTATGCTCTCTATACCGCCGGCATTGCTTGATGTTCTTCATCT
TACTGAGAACACGCAAGTTGGTTTGGCTGTTGATAATGGACAATTGGTTGTGAAGCCACAGACATTTCCAAGTTACACTC
TTGATGAATTGTTAGCTCAATGTCATCTTTCAGATGATTTCTCTGATGAGGATATGGAGTGGTTTGATGCTAAACCTGTT
GGTAGAGAGCTCTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7