164347

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 95701..96227 Replicon plasmid pRHBSTW-00119_2
Accession NZ_CP056853
Organism Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV078_RS24265 Protein ID WP_000323025.1
Coordinates 95701..95988 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV078_RS24270 Protein ID WP_000534858.1
Coordinates 95988..96227 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV078_RS24235 91133..91408 + 276 WP_057064204.1 hypothetical protein -
HV078_RS24240 91462..91818 + 357 WP_165410135.1 hypothetical protein -
HV078_RS24245 91887..93905 + 2019 WP_057064206.1 ParB/RepB/Spo0J family partition protein -
HV078_RS24250 93941..94375 + 435 WP_057064207.1 conjugation system SOS inhibitor PsiB -
HV078_RS24255 94372..95094 + 723 WP_057064208.1 plasmid SOS inhibition protein A -
HV078_RS24260 95097..95423 + 327 WP_057102321.1 hypothetical protein -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
HV078_RS24265 95701..95988 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV078_RS24270 95988..96227 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV078_RS24275 96490..97413 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV078_RS24280 97613..98185 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV078_RS24285 98692..99852 + 1161 WP_032949009.1 IS30-like element ISCfr4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..177615 177615
- flank IS/Tn - - 98692..99852 1160


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T164347 WP_000323025.1 NZ_CP056853:c95988-95701 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T164347 NZ_CP072899:c297351-297019 [Bartonella henselae]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT164347 WP_000534858.1 NZ_CP056853:c96227-95988 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT164347 NZ_CP072899:c296911-296684 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References