Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 95701..96227 Replicon plasmid pRHBSTW-00119_2
Accession NZ_CP056853
Organism Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV078_RS24265 Protein ID WP_000323025.1
Coordinates 95701..95988 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV078_RS24270 Protein ID WP_000534858.1
Coordinates 95988..96227 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV078_RS24235 91133..91408 + 276 WP_057064204.1 hypothetical protein -
HV078_RS24240 91462..91818 + 357 WP_165410135.1 hypothetical protein -
HV078_RS24245 91887..93905 + 2019 WP_057064206.1 ParB/RepB/Spo0J family partition protein -
HV078_RS24250 93941..94375 + 435 WP_057064207.1 conjugation system SOS inhibitor PsiB -
HV078_RS24255 94372..95094 + 723 WP_057064208.1 plasmid SOS inhibition protein A -
HV078_RS24260 95097..95423 + 327 WP_057102321.1 hypothetical protein -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
- 95472..95596 + 125 NuclAT_0 - -
HV078_RS24265 95701..95988 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV078_RS24270 95988..96227 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV078_RS24275 96490..97413 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV078_RS24280 97613..98185 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV078_RS24285 98692..99852 + 1161 WP_032949009.1 IS30-like element ISCfr4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..177615 177615
- flank IS/Tn - - 98692..99852 1160


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T164347 WP_000323025.1 NZ_CP056853:c95988-95701 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T164347 NZ_CP072899:c297351-297019 [Bartonella henselae]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT164347 WP_000534858.1 NZ_CP056853:c96227-95988 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT164347 NZ_CP072899:c296911-296684 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References