Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4829004..4829620 Replicon chromosome
Accession NZ_CP056852
Organism Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HV078_RS22910 Protein ID WP_003028682.1
Coordinates 4829004..4829378 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HV078_RS22915 Protein ID WP_043018956.1
Coordinates 4829378..4829620 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV078_RS22895 4826507..4827409 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV078_RS22900 4827406..4828041 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV078_RS22905 4828038..4828967 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV078_RS22910 4829004..4829378 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HV078_RS22915 4829378..4829620 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HV078_RS22920 4829826..4830734 + 909 WP_032950680.1 alpha/beta hydrolase -
HV078_RS22925 4830885..4831826 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV078_RS22930 4831871..4832308 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV078_RS22935 4832305..4833177 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV078_RS22940 4833171..4833770 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T164346 WP_003028682.1 NZ_CP056852:c4829378-4829004 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T164346 NZ_CP072899:c296700-296431 [Bartonella henselae]
TTGGCTTGGACGATTAGATATGAAAAAAAAGCTCTTAGTTTTTTAAAAAAATGCGATAAAAAAGAAGCACGGCGGATTGT
TGATTTTTTAGATCAACACGTTGCTCCTCTTGAAGATGTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCTCAGGTT
TATGGAGATATCGTGTAGGAGATTACAGGATACTTTGTGAACTCTATGATAAGGAGCTTGTCGTATTAGTTTTGGCTGTT
GGACATAGAAAAAATATATATAAAGGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT164346 WP_043018956.1 NZ_CP056852:c4829620-4829378 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT164346 NZ_CP072899:c296911-296684 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References