Detailed information of TA system TA164345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4676469..4677135
• Replicon: chromosome
• Accession: NZ_CP056852
• Organism: Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8B5QBX6
• Locus tag: HV078_RS22220
• Protein ID: WP_032948292.1
• Coordinates: 4676776..4677135 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HV078_RS22215
• Protein ID: WP_003031625.1
• Coordinates: 4676469..4676786 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV078_RS22185	4672137..4672961	+	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HV078_RS22190	4673029..4674252	+	1224	WP_032948296.1	L-sorbose 1-phosphate reductase	-
HV078_RS22195	4674254..4675066	+	813	WP_032948295.1	shikimate 5-dehydrogenase	-
HV078_RS22200	4675118..4675474	-	357	WP_032948294.1	hypothetical protein	-
HV078_RS22205	4675616..4675777	+	162	WP_003841416.1	phage protein	-
HV078_RS22210	4676181..4676459	+	279	WP_032948293.1	putative addiction module antidote protein	-
HV078_RS22215	4676469..4676786	-	318	WP_003031625.1	helix-turn-helix domain-containing protein	Antitoxin
HV078_RS22220	4676776..4677135	-	360	WP_032948292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV078_RS22225	4677349..4678038	+	690	WP_003841421.1	dipeptidase PepE	-
HV078_RS22230	4678104..4679735	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13461.47 Da
• Isoelectric Point: 10.2555

>T164345 WP_032948292.1 NZ_CP056852:c4677135-4676776 [Citrobacter freundii]
MTKPLYWVGHARKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVAGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T164345 NZ_CP072899:264622-264894 [Bartonella henselae]
ATGAAGCTCGTTTGGACACGGATAGCGCATTACGATCGTCAAAAGATACGGGAATATATCGCGCAAGAAAATCCTTCTGC
TGCATTAGTATTCGACAAGCTTTTATCTAAAAAAGTAGAAAGACTCATTAGCTTTCCTAATCTTGGTCGTGTTGGCCGAA
TTAGGAATACGCGCGAGCTTATTGTGCGTAAGAATTATATTATGATTTATGATGTTTCTAATGGCACTATACGGGTTTTG
CGCATACTTCACGCAAAACAAAAGTGGCCATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11522.46 Da
• Isoelectric Point: 9.0005

>AT164345 WP_003031625.1 NZ_CP056852:c4676786-4676469 [Citrobacter freundii]
MSHEVEASSGNVYADLGVYDAEQMQLKAQLAMVIGEILQRKGLTQQQSAQLLGMTQPKLSNLLRGQFRGISEVKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT164345 NZ_CP072899:264335-264625 [Bartonella henselae]
ATGGCTGAAACAACATTTACCTTTCGCGTTGATGATGCATTAAAAAGTGAATTTTCTCAGGCTGCAAAAGCATGTGATCG
ATCTGCTGCACAGCTCTTACGGGATTACATGCGTGATATTGTAAAAGAACAAAAAGAAAAGATAGCGCATGAATTATGGT
TTCAGGAACAAGTCCAACTTGGATTAAATTCTGCCAACGCAGGTGATGTAATACCTTTTGAAGAAATTGAAACAGAAGCA
CAAGCATGGCGCTTTGAAATACAACGCAAGCTGAAGACATCAGATTCATGA

Structures

Toxin

• AlphaFold DB: A0A8B5QBX6