Detailed information of TA system TA164331

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 773244..773997
• Replicon: chromosome
• Accession: NZ_CP056852
• Organism: Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: HV078_RS03720
• Protein ID: WP_016154457.1
• Coordinates: 773244..773621 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV078_RS03725
• Protein ID: WP_162838704.1
• Coordinates: 773671..773997 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV078_RS03695	769388..771247	+	1860	WP_032950101.1	bifunctional glutathionylspermidine amidase/synthase	-
HV078_RS03700	771286..771585	-	300	WP_032950098.1	hypothetical protein	-
HV078_RS03705	771597..772442	-	846	Protein_726	DUF4942 domain-containing protein	-
HV078_RS03710	772523..772726	-	204	WP_009652505.1	DUF957 domain-containing protein	-
HV078_RS03715	772756..773247	-	492	WP_009652438.1	hypothetical protein	-
HV078_RS03720	773244..773621	-	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
HV078_RS03725	773671..773997	-	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV078_RS03730	774054..774275	-	222	WP_009652392.1	DUF987 domain-containing protein	-
HV078_RS03735	774289..774771	-	483	WP_016154455.1	DNA repair protein RadC	-
HV078_RS03740	774783..775001	-	219	WP_009652503.1	hypothetical protein	-
HV078_RS03745	775012..775473	-	462	WP_045890390.1	antirestriction protein	-
HV078_RS03750	775502..775756	-	255	WP_009652519.1	hypothetical protein	-
HV078_RS03755	775756..776574	-	819	WP_009652424.1	DUF945 domain-containing protein	-
HV078_RS03760	776678..776911	-	234	WP_009652474.1	DUF905 domain-containing protein	-
HV078_RS03765	776967..777188	-	222	WP_032950095.1	hypothetical protein	-
HV078_RS03770	777270..777782	-	513	WP_032950094.1	DUF4234 domain-containing protein	-
HV078_RS03775	777831..778301	-	471	WP_009652431.1	hypothetical protein	-
HV078_RS03780	778348..778659	-	312	WP_009652410.1	hypothetical protein	-
HV078_RS03785	778672..778938	-	267	WP_032950091.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T164331 WP_016154457.1 NZ_CP056852:c773621-773244 [Citrobacter freundii]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T164331 NZ_CP072897:1595396-1595710 [Bartonella henselae]
ATGATACATAAAAGAACGAATGAAGGTGACTTGGTAATTGAATCTTTTGCGGATAAGCGGTGTAAAGATCTTTTAGAAGG
CAATCCACCTAAAGGTTTTCCCACAACTCTCGTGCGTATAGTCCAACGAAAATTATTTATGCTGGATAAAGCAGTTGATC
TCAAAGATTTACGCAGTCCTCCGGGTAATCGTTTAGATGCATTGAAAGGAGAGCGTAAAGGTCAATATTCTATTCGTATT
AATGACCAGTTTCGCATTTGTTTTGAGTGGCGTTCTAATGGTGCCTATGAAGTTGAAATCATCGATTATCATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT164331 WP_162838704.1 NZ_CP056852:c773997-773671 [Citrobacter freundii]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT164331 NZ_CP072897:1595727-1596023 [Bartonella henselae]
ATGAAAAATTATATTGCTATTCATCCCGGAGAAATTTTACGGGAAGAATATTTAAAAGAATATGCTCTCTCTGCTTATGC
TCTTGCCAAAGCCTTGAATGTTCCACGCACTCGGATAGAACGTATTGTAGCAGAAAATAGTCCGGTAACTCCTGATACAG
CACTACGATTAGCCTATTTTTTTGATACAACAGCTGAATTCTGGCTTAACATGCAAGCTGCTTACGACGTTAGTGTCTTA
CAAGCTGAAAAAGCAGATGAATTTTCCAAAATTAGTAAATTTGAATGTAGGGTTTAA

Structures

Toxin

• AlphaFold DB: R8WN26