Detailed information of TA system TA164330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 258846..259432
• Replicon: chromosome
• Accession: NZ_CP056852
• Organism: Citrobacter freundii strain RHBSTW-00119

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: HV078_RS01230
• Protein ID: WP_032937237.1
• Coordinates: 259064..259432 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: HV078_RS01225
• Protein ID: WP_032937236.1
• Coordinates: 258846..259067 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV078_RS01200	254645..255571	+	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV078_RS01205	255568..256845	+	1278	WP_032950432.1	branched chain amino acid ABC transporter permease LivM	-
HV078_RS01210	256842..257609	+	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV078_RS01215	257627..258340	+	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV078_RS01220	258443..258724	+	282	WP_003023442.1	hypothetical protein	-
HV078_RS01225	258846..259067	+	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV078_RS01230	259064..259432	+	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV078_RS01235	259676..260992	+	1317	WP_003837839.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV078_RS01240	261093..261980	+	888	WP_032950430.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV078_RS01245	261977..262822	+	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV078_RS01250	262825..263895	+	1071	WP_032950429.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	255568..264635	9067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T164330 WP_032937237.1 NZ_CP056852:259064-259432 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T164330 NZ_CP072897:1562954-1563277 [Bartonella henselae]
ATGCGTGGATCTCTCGTCACAATAGCTATGCAAGGCGATTTTGGTAAACCAAGACCTGCGTTAATCATTCAAGCCAATCA
ATTTAGTGAACATACAAGTGTAACGGTTTTACCCATTACTAGCACACTTGTTGCAGCGCCATTACTTCGCATTACGATTC
AGCCAAACGCTAAAAATGGTTTGCAAAAACCTTCGCAAGTCATGATTGATAAGATTATGACGGTAAGATGTGAAAAAGTT
AGTCCAGCTTTCGGCTCCATTCATGCAGATAAAATGGTAGAAATTGAACGCTGTTTAGCTGTATTTTTAGGAATAGTAAA
ATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT164330 WP_032937236.1 NZ_CP056852:258846-259067 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT164330 NZ_CP072897:1562730-1562954 [Bartonella henselae]
ATGGCTACAATGCACGTCAATGAGCGCGTTCAAAAACATCGCAATGCACAAAGAAAAGCAGGTTTGCGCTTAATGCAAAT
TTGGGTACCAGATACTCGTCAACCAAACTTTGCAGAAGAGTGTCGCCGCCAATGTCGCTTAGTCGCAAAAATGGATAAAA
CAGATACATCTATGCAGTTGTTAATGGATCAATCTTTAATGGATGTTGATGGCTGGACAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092