Detailed information of TA system TA164315

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 781602..782281
• Replicon: chromosome
• Accession: NZ_CP056850
• Organism: Citrobacter freundii strain RHBSTW-00120

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV079_RS03855
• Protein ID: WP_181637544.1
• Coordinates: 781940..782281 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0F6RHY7
• Locus tag: HV079_RS03850
• Protein ID: WP_046495841.1
• Coordinates: 781602..781919 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV079_RS03810	777161..777994	+	834	WP_181637542.1	DUF945 domain-containing protein	-
HV079_RS03815	778196..778900	+	705	WP_181637543.1	WYL domain-containing protein	-
HV079_RS03820	778897..779511	+	615	WP_103798305.1	hypothetical protein	-
HV079_RS03825	779600..780010	+	411	WP_103798304.1	hypothetical protein	-
HV079_RS03830	780088..780324	+	237	WP_046495830.1	DUF905 domain-containing protein	-
HV079_RS03835	780403..780861	+	459	WP_053463660.1	antirestriction protein	-
HV079_RS03840	780877..781353	+	477	WP_046495838.1	RadC family protein	-
HV079_RS03845	781362..781583	+	222	WP_000691995.1	DUF987 domain-containing protein	-
HV079_RS03850	781602..781919	+	318	WP_046495841.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV079_RS03855	781940..782281	+	342	WP_181637544.1	TA system toxin CbtA family protein	Toxin
HV079_RS03860	782397..783230	+	834	WP_046495848.1	DUF4942 domain-containing protein	-
HV079_RS03870	783533..784039	+	507	WP_115257458.1	G/U mismatch-specific DNA glycosylase	-
HV079_RS03875	784085..785929	-	1845	WP_096755616.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12956.93 Da
• Isoelectric Point: 10.7238

>T164315 WP_181637544.1 NZ_CP056850:781940-782281 [Citrobacter freundii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRGESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T164315 NZ_CP072890:36713-37576 [Enterococcus raffinosus]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAAAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACGAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTAAG
CGATCAAGGCTATAACTTGGTGATTGAAGGTACAGGACGAACAACAGACGTTCCCATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCAAAAATACCTAAGCTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11869.49 Da
• Isoelectric Point: 6.6245

>AT164315 WP_046495841.1 NZ_CP056850:781602-781919 [Citrobacter freundii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT164315 NZ_CP072890:36439-36711 [Enterococcus raffinosus]
ATGGCAGTTACTTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGCTATTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6RHY7