Detailed information of TA system TA164312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 252701..254324
• Replicon: chromosome
• Accession: NZ_CP056850
• Organism: Citrobacter freundii strain RHBSTW-00120

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A7D6U035
• Locus tag: HV079_RS01170
• Protein ID: WP_020833804.1
• Coordinates: 252999..254324 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5V9NJV0
• Locus tag: HV079_RS01165
• Protein ID: WP_024143021.1
• Coordinates: 252701..252994 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV079_RS01140	247794..248717	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
HV079_RS01145	248980..249219	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	-
HV079_RS01150	249219..249506	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	-
HV079_RS01160	250948..252300	+	1353	WP_049827791.1	hypothetical protein	-
HV079_RS01165	252701..252994	+	294	WP_024143021.1	helix-turn-helix transcriptional regulator	Antitoxin
HV079_RS01170	252999..254324	+	1326	WP_020833804.1	type II toxin-antitoxin system HipA family toxin	Toxin
HV079_RS01175	254385..254591	+	207	WP_000134171.1	hypothetical protein	-
HV079_RS01180	254761..255255	+	495	WP_071685143.1	hypothetical protein	-
HV079_RS01185	255255..256853	+	1599	Protein_233	transcriptional antiterminator	-
HV079_RS01190	257017..257919	+	903	WP_020833802.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	229436..299646	70210
	-	inside	IScluster/Tn	-	-	242219..250513	8294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-70)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50307.23 Da
• Isoelectric Point: 6.0252

>T164312 WP_020833804.1 NZ_CP056850:252999-254324 [Citrobacter freundii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T164312 NZ_CP072888:c832919-832734 [Enterococcus raffinosus]
ATGGATGCTAAGGAGCTAGCCAAGATTCTTAAAAACGACGGTTGGTATTTTGATTCTCAACGTGGAAGTCATCGCTACTA
CAAACATCCCATAAAAAAGGGCAAAGTACCTGTCCCATTCCACAGTCACAAAGACATCCCTATTGGAACACTCAACAACA
TACTTAAACAAGCTGGCTTAAAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10990.39 Da
• Isoelectric Point: 9.9492

>AT164312 WP_024143021.1 NZ_CP056850:252701-252994 [Citrobacter freundii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKLNSPARQEDW

Nucleotide

• Length: 294 bp

>AT164312 NZ_CP072888:c832680-832294 [Enterococcus raffinosus]
GTGGTCATTTATTATGCTGTGTTCGATTTTGCAGATGATGGGATCAATGTTGTTTTTCCAGATTTGAACAATGCGGCTAC
ATTTGGTGAAAATATGCATGAGGCCCTCTATATGGCTAAAGATTTACTAGCAGGTTGGCTGATTGATGCTGAGGATGAAA
AGCAAGCTTTTCCTTCACCTACTGATCACCGAAGTCTGAGTGTAGCAGCTGGAAATTTATTGATCCCAATTGAAGTTGAT
TTATCCTTTTACCGTAAAAAATTTGAAAGTAAGCCTATCAAAAAAACATTAACTATTCCAAAATATTTAAATGATTTAGG
CAATGAAGCAGGAATCAACTTCTCAGCTACACTAACTGAAGCATTGAAGGAAAAATTAGAAGTTTAG

Structures

Toxin

• AlphaFold DB: A0A7D6U035

Antitoxin

• AlphaFold DB: A0A5V9NJV0