Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4194267..4195020 Replicon chromosome
Accession NZ_CP056839
Organism Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)


Gene name yeeV Uniprot ID A0A097QYR4
Locus tag HV083_RS20175 Protein ID WP_025799003.1
Coordinates 4194267..4194644 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7T0E0C6
Locus tag HV083_RS20180 Protein ID WP_072269511.1
Coordinates 4194694..4195020 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV083_RS20130 4189417..4189617 - 201 WP_001550179.1 hypothetical protein -
HV083_RS20135 4189604..4189831 - 228 WP_181621198.1 DUF2724 domain-containing protein -
HV083_RS20140 4189839..4190348 - 510 WP_096185558.1 phage regulatory CII family protein -
HV083_RS20145 4190379..4190642 - 264 WP_024138324.1 hypothetical protein -
HV083_RS20150 4190777..4191364 + 588 WP_181621202.1 phage repressor protein CI -
HV083_RS20155 4191364..4192407 + 1044 WP_181621211.1 tyrosine-type recombinase/integrase -
HV083_RS20160 4192620..4193465 - 846 WP_049016442.1 DUF4942 domain-containing protein -
HV083_RS20165 4193547..4193750 - 204 WP_025799007.1 DUF957 domain-containing protein -
HV083_RS20170 4193779..4194270 - 492 WP_045888216.1 hypothetical protein -
HV083_RS20175 4194267..4194644 - 378 WP_025799003.1 TA system toxin CbtA family protein Toxin
HV083_RS20180 4194694..4195020 - 327 WP_072269511.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV083_RS20185 4195077..4195298 - 222 WP_045888217.1 DUF987 domain-containing protein -
HV083_RS20190 4195312..4195791 - 480 WP_025798999.1 DNA repair protein RadC -
HV083_RS20195 4195803..4196234 - 432 WP_088903392.1 antirestriction protein -
HV083_RS20200 4196457..4199612 - 3156 WP_077267777.1 AIDA repeat-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4157831..4223765 65934


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(4-103)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13993.97 Da        Isoelectric Point: 6.8515

>T164307 WP_025799003.1 NZ_CP056839:c4194644-4194267 [Citrobacter freundii]
MQTQPVPPTQEASPRPSPVEIWLRLLSHLLDRHYGLTLNDTPFCNDGVIQEHIDAGISLCDAVNFIVEKYALIRTDRHGF
SAETQSPLISSIDILRARKACGLMARNGYRLVTDITTGKYSEVTR

Download         Length: 378 bp

>T164307 NZ_CP072882:c168802-167939 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACACCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGCACAGGACGAACAACAGACGTTCCTATTCAAACAGCCACAATGCTACAAG
CCAAAGGTTATGAAACAAAAACGTACGCCATGGCAGTACCCAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12016.71 Da        Isoelectric Point: 6.4674

>AT164307 WP_072269511.1 NZ_CP056839:c4195020-4194694 [Citrobacter freundii]
IAEPWWGLKRDITPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELNPRHQHGVTLYA
KGLTCNADTLGSCGYVYIAIYPTPATTE

Download         Length: 327 bp

>AT164307 NZ_CP072882:c169076-168804 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097QYR4

Antitoxin

Source ID Structure
AlphaFold DB A0A7T0E0C6

References