Detailed information of TA system TA164296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2464735..2465374
• Replicon: chromosome
• Accession: NZ_CP056839
• Organism: Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV083_RS11890
• Protein ID: WP_003020221.1
• Coordinates: 2465198..2465374 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV083_RS11885
• Protein ID: WP_127785375.1
• Coordinates: 2464735..2465151 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV083_RS11865	2459781..2460725	-	945	WP_048217772.1	ABC transporter permease	-
HV083_RS11870	2460726..2461742	-	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
HV083_RS11875	2461759..2462904	-	1146	WP_057064386.1	ABC transporter substrate-binding protein	-
HV083_RS11880	2463233..2464642	-	1410	WP_016150280.1	PLP-dependent aminotransferase family protein	-
HV083_RS11885	2464735..2465151	-	417	WP_127785375.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV083_RS11890	2465198..2465374	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV083_RS11895	2465638..2467041	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
HV083_RS11900	2467041..2468051	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV083_RS11905	2468051..2468176	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV083_RS11910	2468366..2468596	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T164296 WP_003020221.1 NZ_CP056839:c2465374-2465198 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T164296 NZ_CP072873:c1072005-1071817 [Pseudomonas fluorescens]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGCAAGAGGCGGGTTGGTTGCTGGATCGGGTCACGGGCAGTCATCACATTTT
TACTCACCGCTATAACCCGTACACGATCCCGGTGCCGCACCCCAAGAAGGACCTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15112.33 Da
• Isoelectric Point: 4.5610

>AT164296 WP_127785375.1 NZ_CP056839:c2465151-2464735 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKDLALTML

Nucleotide

• Length: 417 bp

>AT164296 NZ_CP072873:c1071805-1071401 [Pseudomonas fluorescens]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCACCGGTATTCAGATCCCCGATATTCCAGGCGCGGT
CACCGCCGGGGACAGTTTCGAAGAGGCGTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAAATCGCCGCCG
AAGGTGGGCCGATTCCGATGCCCACGTCGGTGGCCAATCATCACGCCCATGAAGACTACGCCGGGATGGGCTGGGGCATG
CTCGAGCTGGACGTCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAACGTGACCTTGCCCGGTTATGTCATCCAGCGCAT
CGACCGTTATGTGCGCGAGCACAAGGTCAAAAGCCGCTCGTCATTCCTGGCGGACGCGGCCTTGGAGAAGCTGGTGCGCT
CCTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36