Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1254610..1255230 Replicon chromosome
Accession NZ_CP056839
Organism Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV083_RS05905 Protein ID WP_002892050.1
Coordinates 1254610..1254828 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV083_RS05910 Protein ID WP_003021733.1
Coordinates 1254856..1255230 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV083_RS05875 1249837..1250841 + 1005 WP_047359309.1 IS110-like element IS4321 family transposase -
HV083_RS05880 1251040..1252620 - 1581 WP_048219473.1 EAL domain-containing protein -
HV083_RS05885 1252809..1253069 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV083_RS05890 1253071..1253211 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV083_RS05895 1253290..1253760 - 471 WP_003021724.1 YlaC family protein -
HV083_RS05900 1253877..1254428 - 552 WP_048219472.1 maltose O-acetyltransferase -
HV083_RS05905 1254610..1254828 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV083_RS05910 1254856..1255230 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV083_RS05915 1255718..1258867 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV083_RS05920 1258890..1260083 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1249837..1250841 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T164295 WP_002892050.1 NZ_CP056839:c1254828-1254610 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T164295 NZ_CP072871:74544-74945 [Rhizobium sp. NLR16a]
ATGCCGCTCTACATGCTTGACACAAACATGTGCATCTATCTGATGAAGGAGCAGCCGCCGCAAGTCGCACGACGTTTTGC
TCAATGCCGCGTTGGCGATGTCGTCATGTCTGCCATTACGTTCGCGGAACTCGAATATGGCGTTTCTGCCTCGAAGGATG
CAGAAAGGGAACGGTCGAACCTAGAGTATCTGATCGAGGCAATCCCTGTCGCCCCGTTCGATAGTGATGCGGCACGGGCC
TACGGACCTCTCAGGTTCGCGACACGTGACCGGAAAAAGGATCACTTGGACAAACTGATAGCCGCTCATGCGGTTTCGCT
CAAGGCTGTCCTCGTGACGAATAATGTGCGGGACTTTACGGTTTACCAGGACTTGCGCGTCGAAAACTGGCTGTTCCCAT
GA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT164295 WP_003021733.1 NZ_CP056839:c1255230-1254856 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT164295 NZ_CP072871:74308-74544 [Rhizobium sp. NLR16a]
ATGCATACGACAAAAGTATTCAAGAACGGCAACTCTCAAGCCGTGAGAATTCCCGCCGATTTGGCTTACAGCCAGACCGA
GATGGAGCTTGAAATCGAGCGCATTGGTGACGAGCTCCGCATCCGCCCGCTAAAACCGTCCCTGGAGGGCGTACTGGCGC
GTTTCGCAAGATTTGACGACTCGTTCATGGCGGAGGGGCGCGGTGATCAGGAGCAGGCCGAACGGGATAGGCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References