164294

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 1054635..1055314 Replicon chromosome
Accession NZ_CP056839
Organism Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV083_RS04930 Protein ID WP_003031349.1
Coordinates 1054635..1054976 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV083_RS04935 Protein ID WP_003031347.1
Coordinates 1054997..1055314 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV083_RS04905 1050882..1051493 + 612 WP_181621424.1 hypothetical protein -
HV083_RS04910 1051493..1052560 + 1068 WP_181621427.1 hypothetical protein -
HV083_RS04915 1052563..1052904 + 342 WP_181621429.1 hypothetical protein -
HV083_RS04920 1052917..1053648 + 732 WP_181621431.1 phage baseplate protein -
HV083_RS04925 1053910..1054098 + 189 WP_181621434.1 hypothetical protein -
HV083_RS04930 1054635..1054976 - 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV083_RS04935 1054997..1055314 - 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV083_RS04940 1055332..1055553 - 222 WP_003031346.1 DUF987 domain-containing protein -
HV083_RS04945 1055562..1056038 - 477 WP_003031345.1 RadC family protein -
HV083_RS04950 1056054..1056515 - 462 WP_003031344.1 antirestriction protein -
HV083_RS04955 1056529..1056720 - 192 Protein_967 DUF905 family protein -
HV083_RS04960 1057224..1059221 + 1998 WP_016245729.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1039180..1056515 17335


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T164294 WP_003031349.1 NZ_CP056839:c1054976-1054635 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T164294 NZ_CP072870:208107-208400 [Rhizobium sp. NLR16a]
ATGCGCTTTGACCTTTCAGTCGAAGCGGAAGAGGACATCATTGCGATCGCCGAACAAGGAGTGCGCATGTTCGGAGCCGC
ACAGGCTAGGCAGTATCATGACGAGTTGTTTACCGTGCTTGATCTGATTGCGGCCAATCCGCGAATGGCGCGTGAGCGAG
AGGAGATTTCGCCGCCAGTTAGAGTTCATCCGTTCAAAGCCCATCTCATTGTCTACCGGATCGAGGAGCACGGAGCTATA
TTCGTGATCCGAATACGCCATGGACACGAAGACTGGGCCAGCGATCCTGCTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT164294 WP_003031347.1 NZ_CP056839:c1055314-1054997 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT164294 NZ_CP072870:207853-208107 [Rhizobium sp. NLR16a]
ATGGCTACGATGAACGTATCTCTTCCGGACCCGATGAAAGATTGGGTTGAGGCACAAGCAAAGAGCGGGCGATATTCCAA
TGCGAGTGACTATGTGCGCGACCTGATCCGGAGGGACCAAATTCGCAGTGACAAGGTTGCCGTCATGCAGCGTTTCGTTG
ATGACGGTTTTAAAAGCGGTATTGGCAGCCGCTCGAAGGACGAACTCTTTGCCGCGGCAGTGGCACGTGCAGAAACAACG
CGCGGCGGTAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References