Detailed information of TA system TA164293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 702240..702816
• Replicon: chromosome
• Accession: NZ_CP056839
• Organism: Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV083_RS03315
• Protein ID: WP_003019170.1
• Coordinates: 702240..702527 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV083_RS03320
• Protein ID: WP_003019167.1
• Coordinates: 702514..702816 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV083_RS03290	697593..699005	-	1413	WP_048219782.1	PLP-dependent aminotransferase family protein	-
HV083_RS03295	699183..699347	+	165	WP_003019180.1	DUF1127 domain-containing protein	-
HV083_RS03300	699495..699959	+	465	WP_003019178.1	MarR family transcriptional regulator	-
HV083_RS03305	699966..701033	+	1068	WP_032948464.1	HlyD family secretion protein	-
HV083_RS03310	701023..702087	+	1065	WP_048219780.1	DUF2955 domain-containing protein	-
HV083_RS03315	702240..702527	+	288	WP_003019170.1	BrnT family toxin	Toxin
HV083_RS03320	702514..702816	+	303	WP_003019167.1	BrnA antitoxin family protein	Antitoxin
HV083_RS03325	702880..703836	-	957	WP_003019163.1	GTPase	-
HV083_RS03330	703849..704043	-	195	WP_003830038.1	YbdD/YjiX family protein	-
HV083_RS03335	704154..706304	-	2151	WP_048219778.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11157.53 Da
• Isoelectric Point: 7.0746

>T164293 WP_003019170.1 NZ_CP056839:702240-702527 [Citrobacter freundii]
MPMEFEWDANKAQSNLRKHGVRFEDAVLVFDDPQHLSRQDRHENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T164293 NZ_CP072869:c269506-268637 [Rhizobium sp. NLR16a]
ATGGCTAAAGAAATCAGAAACGTCGGCGCCTCGGTGCGCGCCCGCCTGTTGCAACTCGCCAAGGCAAGCGGGCAAAGCTT
CGATCTCGTCCTGACGCGTTTCGCCCTTGAACGGTTATTGTTCCGGCTTAGCCAGTCGCCCCACGCCGATCGCTTCGTTC
TAAAAGGGGCGATGTTGATGATGAGTTGGTTCGACAATCCGCACCGAGGCACGCGCGACCTGGATCTTTTGGGTTTCGGT
GATCCGAGTCCGGACCCGATGCTTGAGACATTTCGGGAAATTCTGGCGCAAGAGGCCGACGATGGCGTTACGTTCGACGC
TGATACGCTGCATGTGGATCGTATTCGCGAGGCACTGGATTATGGTGGGCTCAGGCTGCGGGTGATCGCTTCGATCAGCG
GAGCCCGGATCAACCTGACCATCGACATCGGTTTTGGCGATGCGCTTGAGCCTGGGGTGGAGGTTTTGGATTATCCTTCC
ATGCTCGATTTTCCGATGCCGCGGCTTCGGGCCTATGCGCGAGAAACGGTCATTGCCGAGAAGTTCCAGGCAATGGTGAT
GCTGGGGCGAGTAAACAGCCGCATGAAGGATTTCTACGACATCTGGATCCTCAGCAGGTCTTTCGACTTCAGCGATGATC
GGCTGGCGCGCGCAATAGCCGCTACCTTTGATCGCCGCGAGACACCGATCCCCATTGACCTGCCGGACGCCCTAACGGAC
GCCTTTGCCAAGGACCAGCAAAAGCAGCGACAATGGCGTGCTTTCATTGAAGGCGTTGCACACAATCCTGGCGATCTGAT
CGACGTCATCGCGCAGATTGCCACATTCCTGATGCCGCACGCGGTTGCGGCTGCGAGGCTGGACGAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11462.06 Da
• Isoelectric Point: 10.1293

>AT164293 WP_003019167.1 NZ_CP056839:702514-702816 [Citrobacter freundii]
MSMVKHKRGSVSALSAQHEAELKALANKSDDDIDYSDIPVTEDKQWSEATRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT164293 NZ_CP072869:c270116-269499 [Rhizobium sp. NLR16a]
ATGCCCAGTTCAGTCACGCAACGCCAAATCGCTCGGACCGTGCTAACCGAGCGCGGAATAGCACGTTTCGTCGAACTGCG
GAACGCAGGGGTGACGGCTGCCACCATGAGCCGAATGGAACGAGATGGTGAAGTGCTGCGGCTTGCCCGCGGTCTCTATC
AACTTCCTGATGCGCAGCTCGACGCCAGCCACAGCCTGGCGGAGGTTGCCAAACGCGCGCCCAAAGCCGTTATCTGCCTC
GTTTCGGCATTGGCATTTCACAGCCTGACAGATCAGCTCCCCGGACAAATCTGGCTTGCCATAGGCCGTAAGGACTGGCC
GCCGAAGCTGGAAGCCCCCGCTGTGCGCATCGTGCGCTTCACAGACAGCCTTCTCAACGACAGCGTCGAAACCCATATCA
TTGAAGGCGTTCCTGTGAGGGTCTTTGGAATCGCCAAGACAATTGCCGATTGCTTTCGATATCGCAACAAGATCGGCCTT
TCGGTAGCGATAGAAGGGCTGCAGGAGGCGCTGCGGCAACGAAGGGCTACTCCTGGCGAAATCGCCAACCAGGCCGAACG
CGGTGGCGTTGGCTCTGTGATCCGGCCGTATCTCGAGGCGCTGACCGCCAATGGCTAA