Detailed information of TA system TA164291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 45411..46208
• Replicon: plasmid pRHBSTW-00127_3
• Accession: NZ_CP056836
• Organism: Citrobacter sp. RHBSTW-00127

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV084_RS25720
• Protein ID: WP_172754447.1
• Coordinates: 45705..46208 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HV084_RS25715
• Protein ID: WP_016582626.1
• Coordinates: 45411..45680 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV084_RS25690	41034..41759	-	726	WP_071684184.1	hypothetical protein	-
HV084_RS25695	42041..43096	+	1056	WP_001238819.1	hypothetical protein	-
HV084_RS25700	43166..43921	-	756	WP_052930811.1	DUF2971 domain-containing protein	-
HV084_RS25705	43970..44323	-	354	WP_162199642.1	hypothetical protein	-
HV084_RS25710	44329..44994	-	666	WP_023315962.1	AAA family ATPase	-
HV084_RS25715	45411..45680	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HV084_RS25720	45705..46208	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
HV084_RS25725	46236..46442	-	207	WP_077267659.1	hypothetical protein	-
HV084_RS25730	46393..46644	-	252	WP_004109798.1	hypothetical protein	-
HV084_RS25735	46646..47338	-	693	WP_071684138.1	hypothetical protein	-
HV084_RS25740	47352..47675	-	324	WP_000064173.1	hypothetical protein	-
HV084_RS25745	47750..48538	-	789	WP_071684139.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119392	119392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T164291 WP_172754447.1 NZ_CP056836:45705-46208 [Citrobacter sp. RHBSTW-00127]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T164291 NZ_CP072867:c288876-288493 [Rhizobium sp. NLR16a]
GTGATTCTGGCGGACACTTCGATTTGGATTGATCATTTCCGCCATGCCGATCCAGAGCTGCGCAAGATTATCGAGGATGA
TCGTCTCCTGTGCCATCCGGCTGTGATTGGCGAGTTGGCTCTTGGCAGCCTTCGCAATCGCGGCAGCGTTATCGCTTTCC
TCGCGGCCCAACGCGAAGTCGCCGTCGCCACACACGACGAAGTCATGGTGATGATTGATCGCCACAACATTTTCAGCATG
GGCATTGGCTATACGGATGCCCATTTGCTGGCCTCGGTCCTCCTTGATCGGCGAGCAGCCCTCTGGACCAGAGACAAGCG
TCTGCAGGGAGCAGCCGGAAAGGCGGGCGTTTCACTATACACCCCGGTCGATAGGCGCAGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT164291 WP_016582626.1 NZ_CP056836:45411-45680 [Citrobacter sp. RHBSTW-00127]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT164291 NZ_CP072867:c289073-288873 [Rhizobium sp. NLR16a]
ATGCGATCGACTATCAACCTCGACGACGCTCTCATGCAAAAAGCCAAGGCCCTGACCGGCACAAAAGAAACTGCCGCTCT
CGTCCGCCAAGCGTTGGAGACACTTGTCCGGGTGGAATCCGGAAAACGCCTCATCGCGCTCGGGGGAACCATGCCGGAGG
CAGAGGCAGCCCCACGACGCCGGAGCGCCGTGGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3