Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 95335..96138 Replicon plasmid pRHBSTW-00127_2
Accession NZ_CP056835
Organism Citrobacter sp. RHBSTW-00127

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag HV084_RS25065 Protein ID WP_022652313.1
Coordinates 95608..96138 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV084_RS25060 Protein ID WP_022652312.1
Coordinates 95335..95604 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV084_RS25045 91640..91947 - 308 Protein_101 IS1 family transposase -
HV084_RS25050 92424..93164 + 741 WP_022652310.1 site-specific integrase -
HV084_RS25055 93490..94479 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
HV084_RS25060 95335..95604 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV084_RS25065 95608..96138 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
HV084_RS25070 96270..97163 + 894 WP_161348953.1 IS5 family transposase -
HV084_RS25075 97304..97780 - 477 Protein_107 IS3 family transposase -
HV084_RS25080 97861..98163 - 303 Protein_108 group II intron reverse transcriptase/maturase -
HV084_RS25090 99362..100570 - 1209 Protein_110 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..167459 167459
- inside IScluster/Tn - - 90326..104733 14407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T164290 WP_022652313.1 NZ_CP056835:95608-96138 [Citrobacter sp. RHBSTW-00127]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T164290 NZ_CP072866:155155-155565 [Rhizobium sp. NLR16a]
GTGATTACGCATCTGATCGACACCAATGCCGTGATTGCCTTGATCGGTCGCAAGTCCGACATGCTGCTCTCCCGCGTGAT
GGAGAGCGACGAGGGATCGATCGGCCTCTCCACCGTCGTCATGCATGAACTCTATTACGGCGCCTACAAGAGCGCTAAAA
TTTCCTACAATCTCGAAACCCTGCGCCTGTTCATGGCGGATTTCCCAGCCGTCGGTTTCGAGCGCGAGGATGCGCTGGCA
GCCGGGGAGATCCGCGCCGCATTGGCGGCCAAGGGAAAGCCGATCGGTCCTTACGATGTGCTGATTGCCGCGCAAGCCAG
GAGCCGCGATCTGGTTCTCGTCACCAACAATGTCGGGGAATTCCAGCGCGTCGAAGGCTTGTGCGTCGAGGATTGGACGG
TCGGGCGGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT164290 WP_022652312.1 NZ_CP056835:95335-95604 [Citrobacter sp. RHBSTW-00127]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT164290 NZ_CP072866:154898-155158 [Rhizobium sp. NLR16a]
ATGCCGCAATATGCACGCGTGTTTCAATCCGGCAATTCGCAGGCCGTGCGGCTGCCGAAGGAGTTCCGTTTCGATGTGGA
ACAAGTGGAAGTGACGCGGGAAGGCGATGCCGTCATTCTCCGGCCGCGGGTCGATAGCCGCGTGCAGTGGGCGTCGTTGC
GAAACGCTCTTGAACGCGGGCTCAGCGACGATTTCATGGCACAAGGGCGAGAACAGCCGGAGGATCAGGAGAGGCCTGGT
CTTCAGGGCCTGTTCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References