Detailed information of TA system TA164283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2539905..2540541
• Replicon: chromosome
• Accession: NZ_CP056834
• Organism: Citrobacter sp. RHBSTW-00127

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV084_RS12260
• Protein ID: WP_049259794.1
• Coordinates: 2540353..2540541 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV084_RS12255
• Protein ID: WP_131341011.1
• Coordinates: 2539905..2540321 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV084_RS12235	2534950..2535894	-	945	WP_016153152.1	ABC transporter permease	-
HV084_RS12240	2535895..2536911	-	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HV084_RS12245	2536928..2538073	-	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV084_RS12250	2538403..2539812	-	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
HV084_RS12255	2539905..2540321	-	417	WP_131341011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV084_RS12260	2540353..2540541	-	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV084_RS12265	2540805..2542208	+	1404	WP_047417372.1	cytochrome ubiquinol oxidase subunit I	-
HV084_RS12270	2542208..2543218	+	1011	WP_181640836.1	cytochrome d ubiquinol oxidase subunit II	-
HV084_RS12275	2543218..2543343	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV084_RS12280	2543533..2543763	+	231	WP_016153158.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T164283 WP_049259794.1 NZ_CP056834:c2540541-2540353 [Citrobacter sp. RHBSTW-00127]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T164283 NZ_CP072858:c4185175-4184825 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15034.31 Da
• Isoelectric Point: 4.7119

>AT164283 WP_131341011.1 NZ_CP056834:c2540321-2539905 [Citrobacter sp. RHBSTW-00127]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGAEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT164283 NZ_CP072858:c4185420-4185169 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9