Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4004560..4005239 Replicon chromosome
Accession NZ_CP056809
Organism Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV103_RS19245 Protein ID WP_003031349.1
Coordinates 4004898..4005239 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV103_RS19240 Protein ID WP_003031347.1
Coordinates 4004560..4004877 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV103_RS19205 3999716..4000363 - 648 WP_050489294.1 hypothetical protein -
HV103_RS19210 4000360..4001607 - 1248 WP_032650528.1 hypothetical protein -
HV103_RS19215 4001618..4002709 - 1092 WP_032650530.1 hypothetical protein -
HV103_RS19220 4003164..4003355 + 192 Protein_3768 DUF905 family protein -
HV103_RS19225 4003369..4003830 + 462 WP_003031344.1 antirestriction protein -
HV103_RS19230 4003846..4004322 + 477 WP_003031345.1 RadC family protein -
HV103_RS19235 4004331..4004542 + 212 Protein_3771 DUF987 domain-containing protein -
HV103_RS19240 4004560..4004877 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV103_RS19245 4004898..4005239 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV103_RS19255 4005869..4006333 - 465 WP_003843778.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV103_RS19260 4006526..4007974 - 1449 WP_016149624.1 EAL domain-containing protein -
HV103_RS19265 4008316..4008789 - 474 WP_044702484.1 hypothetical protein -
HV103_RS19270 4008764..4009495 - 732 WP_046669763.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3985211..4017136 31925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T164250 WP_003031349.1 NZ_CP056809:4004898-4005239 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T164250 NZ_CP072850:386667-386954 [Vibrio cholerae MO10]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT164250 WP_003031347.1 NZ_CP056809:4004560-4004877 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT164250 NZ_CP072850:386426-386677 [Vibrio cholerae MO10]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References