Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3762261..3762881 Replicon chromosome
Accession NZ_CP056809
Organism Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV103_RS18115 Protein ID WP_002892050.1
Coordinates 3762663..3762881 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV103_RS18110 Protein ID WP_003021733.1
Coordinates 3762261..3762635 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV103_RS18100 3757407..3758600 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV103_RS18105 3758623..3761772 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV103_RS18110 3762261..3762635 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV103_RS18115 3762663..3762881 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV103_RS18120 3763188..3763739 + 552 WP_003835924.1 maltose O-acetyltransferase -
HV103_RS18125 3763856..3764326 + 471 WP_003021724.1 YlaC family protein -
HV103_RS18130 3764405..3764545 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV103_RS18135 3764547..3764807 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV103_RS18140 3764996..3766549 + 1554 WP_046669815.1 EAL domain-containing protein -
HV103_RS18145 3766601..3766954 - 354 WP_003831033.1 DUF1428 family protein -
HV103_RS18150 3767019..3767648 - 630 WP_016149709.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T164249 WP_002892050.1 NZ_CP056809:3762663-3762881 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T164249 NZ_CP072850:385210-385611 [Vibrio cholerae MO10]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT164249 WP_003021733.1 NZ_CP056809:3762261-3762635 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT164249 NZ_CP072850:385040-385210 [Vibrio cholerae MO10]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References