164248

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3060949..3061539 Replicon chromosome
Accession NZ_CP056809
Organism Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag HV103_RS14790 Protein ID WP_003836692.1
Coordinates 3060949..3061281 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag HV103_RS14795 Protein ID WP_003836694.1
Coordinates 3061282..3061539 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV103_RS14760 3056003..3056731 - 729 WP_023143484.1 hypothetical protein -
HV103_RS14765 3057002..3058033 + 1032 WP_004176137.1 IS630-like element ISEc33 family transposase -
HV103_RS14770 3058229..3058432 + 204 WP_181635498.1 hypothetical protein -
HV103_RS14775 3058505..3059455 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
HV103_RS14780 3059557..3060474 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
HV103_RS14790 3060949..3061281 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV103_RS14795 3061282..3061539 - 258 WP_003836694.1 hypothetical protein Antitoxin
HV103_RS14800 3062153..3066052 + 3900 WP_046670495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3057002..3058033 1031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T164248 WP_003836692.1 NZ_CP056809:c3061281-3060949 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T164248 NZ_CP072850:381375-381707 [Vibrio cholerae MO10]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT164248 WP_003836694.1 NZ_CP056809:c3061539-3061282 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT164248 NZ_CP072850:381694-382008 [Vibrio cholerae MO10]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References