Detailed information of TA system TA164247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2445823..2446462
• Replicon: chromosome
• Accession: NZ_CP056809
• Organism: Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV103_RS11790
• Protein ID: WP_003020221.1
• Coordinates: 2445823..2445999 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV103_RS11795
• Protein ID: WP_123924900.1
• Coordinates: 2446046..2446462 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV103_RS11770	2442601..2442831	-	231	WP_046670718.1	DUF2554 family protein	-
HV103_RS11775	2443021..2443146	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV103_RS11780	2443146..2444156	-	1011	WP_046670717.1	cytochrome d ubiquinol oxidase subunit II	-
HV103_RS11785	2444156..2445559	-	1404	WP_003843730.1	cytochrome ubiquinol oxidase subunit I	-
HV103_RS11790	2445823..2445999	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV103_RS11795	2446046..2446462	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV103_RS11800	2446555..2447964	+	1410	WP_046670716.1	PLP-dependent aminotransferase family protein	-
HV103_RS11805	2448293..2449438	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
HV103_RS11810	2449455..2450471	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
HV103_RS11815	2450472..2451416	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T164247 WP_003020221.1 NZ_CP056809:2445823-2445999 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T164247 NZ_CP072850:c366010-365720 [Vibrio cholerae MO10]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT164247 WP_123924900.1 NZ_CP056809:2446046-2446462 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT164247 NZ_CP072850:c366248-366000 [Vibrio cholerae MO10]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36