Detailed information of TA system TA164235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 246919..247550
• Replicon: chromosome
• Accession: NZ_CP056809
• Organism: Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV103_RS01155
• Protein ID: WP_046671263.1
• Coordinates: 246919..247194 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: HV103_RS01160
• Protein ID: WP_003837808.1
• Coordinates: 247191..247550 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV103_RS01145	242970..245717	+	2748	WP_032937231.1	ribosome-associated ATPase/putative transporter RbbA	-
HV103_RS01150	245717..246841	+	1125	WP_046671262.1	ABC transporter permease	-
HV103_RS01155	246919..247194	+	276	WP_046671263.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV103_RS01160	247191..247550	+	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV103_RS01165	247663..248064	-	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
HV103_RS01170	248114..248692	-	579	WP_003837810.1	4'-phosphopantetheinyl transferase AcpT	-
HV103_RS01175	248755..249804	-	1050	WP_003023393.1	AI-2E family transporter	-
HV103_RS01180	249938..251155	+	1218	WP_032950436.1	MFS transporter	-
HV103_RS01185	251159..251716	-	558	WP_046671264.1	DcrB family lipoprotein	-
HV103_RS01190	251789..252454	-	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10238.90 Da
• Isoelectric Point: 10.7844

>T164235 WP_046671263.1 NZ_CP056809:246919-247194 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIRWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T164235 NZ_CP072848:426284-426586 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT164235 WP_003837808.1 NZ_CP056809:247191-247550 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT164235 NZ_CP072848:426039-426296 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0