Detailed information of TA system TA164219

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3677107..3677764
• Replicon: chromosome
• Accession: NZ_CP056779
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HWQ17_RS17605
• Protein ID: WP_148418554.1
• Coordinates: 3677354..3677764 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V3PWU7
• Locus tag: HWQ17_RS17600
• Protein ID: WP_010435322.1
• Coordinates: 3677107..3677373 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS17580	3672513..3673946	-	1434	WP_148418551.1	6-phospho-beta-glucosidase BglA	-
HWQ17_RS17585	3674063..3674794	-	732	WP_148418661.1	MurR/RpiR family transcriptional regulator	-
HWQ17_RS17590	3675061..3675720	+	660	WP_148418552.1	hemolysin III family protein	-
HWQ17_RS17595	3675832..3676812	-	981	WP_148418553.1	tRNA-modifying protein YgfZ	-
HWQ17_RS17600	3677107..3677373	+	267	WP_010435322.1	FAD assembly factor SdhE	Antitoxin
HWQ17_RS17605	3677354..3677764	+	411	WP_148418554.1	protein YgfX	Toxin
HWQ17_RS17610	3677770..3678291	-	522	WP_148418555.1	flavodoxin FldB	-
HWQ17_RS17615	3678393..3679289	+	897	WP_003863442.1	site-specific tyrosine recombinase XerD	-
HWQ17_RS17620	3679318..3680031	+	714	WP_148418556.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HWQ17_RS17625	3680037..3681770	+	1734	WP_148418557.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16166.11 Da
• Isoelectric Point: 11.7121

>T164219 WP_148418554.1 NZ_CP056779:3677354-3677764 [Enterobacter cloacae]
VVLWQSDLRVSWRSQWMSLLLHGLVAALVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWEIIGAPWMLNAGMMLRLRRVEGGRRQHLWLAADSMDAAEWRDLRRLMMQQPTQE

Nucleotide

• Length: 411 bp

>T164219 NZ_CP072845:c1369740-1368994 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACTGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.02 Da
• Isoelectric Point: 5.1340

>AT164219 WP_010435322.1 NZ_CP056779:3677107-3677373 [Enterobacter cloacae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164219 NZ_CP072845:c1368994-1368824 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: V3PWU7