Detailed information of TA system TA164218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2991405..2992012
• Replicon: chromosome
• Accession: NZ_CP056779
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: HWQ17_RS14265
• Protein ID: WP_057979982.1
• Coordinates: 2991405..2991590 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HWQ17_RS14270
• Protein ID: WP_148422485.1
• Coordinates: 2991605..2992012 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS14250	2986730..2988568	+	1839	WP_148422482.1	selenocysteine-specific translation elongation factor	-
HWQ17_RS14255	2988572..2989456	-	885	WP_148422483.1	ROK family protein	-
HWQ17_RS14260	2989623..2991161	+	1539	WP_148422484.1	aldehyde dehydrogenase AldB	-
HWQ17_RS14265	2991405..2991590	+	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
HWQ17_RS14270	2991605..2992012	+	408	WP_148422485.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HWQ17_RS14275	2992016..2993091	-	1076	Protein_2784	type I restriction enzyme HsdR N-terminal domain-containing protein	-
HWQ17_RS14280	2993522..2993977	+	456	WP_148422486.1	4Fe-4S dicluster domain-containing protein	-
HWQ17_RS14285	2993985..2995238	-	1254	WP_148422487.1	valine--pyruvate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T164218 WP_057979982.1 NZ_CP056779:2991405-2991590 [Enterobacter cloacae]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T164218 NZ_CP072845:511812-512162 [Bacillus subtilis]
TTGATTGTGAAACGAGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15187.17 Da
• Isoelectric Point: 4.5129

>AT164218 WP_148422485.1 NZ_CP056779:2991605-2992012 [Enterobacter cloacae]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDEAFQDARDALTAHFEALFEMDEVLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT164218 NZ_CP072845:511526-511807 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0