Detailed information of TA system TA164215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2328039..2328862
• Replicon: chromosome
• Accession: NZ_CP056779
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HWQ17_RS11105
• Protein ID: WP_148422624.1
• Coordinates: 2328461..2328862 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HWQ17_RS11100
• Protein ID: WP_148422623.1
• Coordinates: 2328039..2328404 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS11055	2323678..2323956	+	279	WP_176678713.1	hypothetical protein	-
HWQ17_RS11060	2323990..2324277	+	288	WP_176678714.1	hypothetical protein	-
HWQ17_RS11065	2324290..2324601	+	312	WP_148422616.1	hypothetical protein	-
HWQ17_RS11070	2324681..2325043	+	363	WP_148422617.1	hypothetical protein	-
HWQ17_RS11075	2325104..2325637	+	534	WP_148422618.1	DUF4234 domain-containing protein	-
HWQ17_RS11080	2325726..2326544	+	819	WP_148422619.1	DUF945 domain-containing protein	-
HWQ17_RS11085	2326813..2327292	+	480	WP_148422620.1	antirestriction protein	-
HWQ17_RS11090	2327304..2327783	+	480	WP_148422621.1	DNA repair protein RadC	-
HWQ17_RS11095	2327795..2328016	+	222	WP_148422622.1	DUF987 domain-containing protein	-
HWQ17_RS11100	2328039..2328404	+	366	WP_148422623.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWQ17_RS11105	2328461..2328862	+	402	WP_148422624.1	TA system toxin CbtA family protein	Toxin
HWQ17_RS11110	2328859..2329350	+	492	WP_148422625.1	hypothetical protein	-
HWQ17_RS11115	2329374..2329565	+	192	WP_148422626.1	DUF957 domain-containing protein	-
HWQ17_RS11120	2329648..2330493	+	846	WP_148422627.1	DUF4942 domain-containing protein	-
HWQ17_RS11125	2330970..2331290	-	321	WP_148422628.1	regulator	-
HWQ17_RS11130	2331459..2332187	+	729	WP_148422629.1	winged helix-turn-helix domain-containing protein	-
HWQ17_RS11135	2332180..2332647	+	468	WP_148422630.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2321394..2332647	11253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-120)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14723.06 Da
• Isoelectric Point: 9.5578

>T164215 WP_148422624.1 NZ_CP056779:2328461-2328862 [Enterobacter cloacae]
MQTLYALPVRKASSRLSPVEILQQLLTYLLERHYGLSLNDTQFGNDCVIQQHIDAGISLADALNFLVEKSELFLVEKSGL
VRINRPGFSIHHQSPFISAIDILRPRKAIGLMQRTGYKAVTCAISGRSSGGLQ

Nucleotide

• Length: 402 bp

>T164215 NZ_CP072844:999776-1000093 [Psychrosphaera aestuarii]
ATGCCTCAATTTTCTCTATATAAAAACAAAGACAAGTCCACATCTAGAGCCTACCCTTTCTTTGTAGATGTTCAATCAGA
CCTGTTGAATAGCCTTGATACAAGGATAGTTATTCCATTGACTTCGTTAGAACTTCTTGAAGGATCGGCCCCAACCCATT
TATGTCCGATCATTCATCTTGAAGAAGGTGATTTTGTAATTCTCACTCAACAGACAACAAGTGTCCCAACTAAGATGCTG
AAAGAGGAAGTGACTGATTTAAGGGCTTTCCGCAATGAGATTATCGGCGCACTCGACTTTTTGATTACGGGCATATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13440.39 Da
• Isoelectric Point: 6.6912

>AT164215 WP_148422623.1 NZ_CP056779:2328039-2328404 [Enterobacter cloacae]
MKNTTPVANYDIAEPWWGLKPTVTPCLGARLVQEGNRLHYLADRASITGTFNNEDLRHLDQAFPVLMKQLELMLVSGELN
PRNQHCVTLYAKGLTCDADTLGSCGYLYIAIYPTSGNSIPR

Nucleotide

• Length: 366 bp

>AT164215 NZ_CP072844:999531-999776 [Psychrosphaera aestuarii]
ATGAGAAATGTACATTCAACACAGCCAACAAAAAAAGCAACAAACCTAAGCTTGAATAGCGAACTATTGGCTGAGGCTAA
AAGGCTTAACATTAACTTATCGGCAACAATGGAAAAAGCGCTTGAGCGAGAAGTCAGCGCTAAGTTGCGTGATGAATGGA
TACAACAAAATGCTGATGCAGTTGAATCTTGCAATAAGTTGACCGAAGAGCACGGATTATTCTCTGATTCATATAGGACG
TTTTAA