Detailed information of TA system TA164213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 959862..960659
• Replicon: chromosome
• Accession: NZ_CP056779
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HWQ17_RS04625
• Protein ID: WP_176610672.1
• Coordinates: 959862..960386 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HWQ17_RS04630
• Protein ID: WP_148421116.1
• Coordinates: 960390..960659 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS04595	955447..956136	+	690	WP_148420880.1	pyrimidine utilization protein B	-
HWQ17_RS04600	956148..956534	+	387	WP_148420882.1	pyrimidine utilization protein C	-
HWQ17_RS04605	956542..957342	+	801	WP_148420884.1	pyrimidine utilization protein D	-
HWQ17_RS04610	957352..957942	+	591	WP_148420886.1	malonic semialdehyde reductase	-
HWQ17_RS04615	957951..958445	+	495	WP_148420888.1	pyrimidine utilization flavin reductase protein F	-
HWQ17_RS04620	958467..959789	+	1323	WP_148420890.1	pyrimidine utilization transport protein G	-
HWQ17_RS04625	959862..960386	-	525	WP_176610672.1	GNAT family N-acetyltransferase	Toxin
HWQ17_RS04630	960390..960659	-	270	WP_148421116.1	DUF1778 domain-containing protein	Antitoxin
HWQ17_RS04635	960733..961638	-	906	WP_148420892.1	DMT family transporter	-
HWQ17_RS04640	961755..961928	-	174	WP_032639520.1	general stress protein	-
HWQ17_RS04645	962320..962916	+	597	WP_148420894.1	NAD(P)H:quinone oxidoreductase	-
HWQ17_RS04650	962935..963162	+	228	WP_148420895.1	YccJ family protein	-
HWQ17_RS04655	963195..964436	-	1242	WP_148420897.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
HWQ17_RS04660	964542..964634	+	93	Protein_921	SDR family oxidoreductase	-
HWQ17_RS04665	964636..965139	-	504	WP_148420899.1	DedA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19799.62 Da
• Isoelectric Point: 7.9525

>T164213 WP_176610672.1 NZ_CP056779:c960386-959862 [Enterobacter cloacae]
VVVNLTIEMLSEGTDYNFEDFDCGEPSLNTFLKEHLVRQHNGRILRGYLLKERHRPHVLGYYTLSGSCFEKTMLPSSTQQ
RRIPYSNVPSVTLGRLAIHKALQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAQRFYLKQGFIPLKGENSNAL
FFPTKSIERLFEQE

Nucleotide

• Length: 525 bp

>T164213 NZ_CP072843:476229-476579 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10263.66 Da
• Isoelectric Point: 4.8952

>AT164213 WP_148421116.1 NZ_CP056779:c960659-960390 [Enterobacter cloacae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAERLRNME

Nucleotide

• Length: 270 bp

>AT164213 NZ_CP072843:475942-476223 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA