Detailed information of TA system TA164200

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3306699..3307363
• Replicon: chromosome
• Accession: NZ_CP056776
• Organism: Enterobacter cloacae strain DSM 30054

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HWQ16_RS15975
• Protein ID: WP_044157892.1
• Coordinates: 3306699..3307025 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0M7FI99
• Locus tag: HWQ16_RS15980
• Protein ID: WP_007869729.1
• Coordinates: 3307046..3307363 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ16_RS15955	3302159..3303394	+	1236	WP_013098178.1	alanine transaminase	-
HWQ16_RS15960	3303450..3303521	-	72	WP_099458937.1	membrane protein YpdK	-
HWQ16_RS15965	3303879..3304799	-	921	WP_169746048.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
HWQ16_RS15970	3305110..3306489	+	1380	WP_013098174.1	diguanylate cyclase	-
HWQ16_RS15975	3306699..3307025	-	327	WP_044157892.1	TA system toxin CbtA family protein	Toxin
HWQ16_RS15980	3307046..3307363	-	318	WP_007869729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWQ16_RS15985	3307377..3307604	-	228	WP_013098172.1	DUF987 domain-containing protein	-
HWQ16_RS15990	3307613..3308089	-	477	WP_013098171.1	RadC family protein	-
HWQ16_RS15995	3308105..3308563	-	459	WP_013098170.1	antirestriction protein	-
HWQ16_RS16000	3308667..3308906	-	240	WP_007869725.1	DUF905 domain-containing protein	-
HWQ16_RS16005	3308930..3309751	-	822	WP_007869723.1	DUF945 domain-containing protein	-
HWQ16_RS16010	3309878..3310138	-	261	WP_007869721.1	hypothetical protein	-
HWQ16_RS16015	3310218..3310490	-	273	WP_007869719.1	hypothetical protein	-
HWQ16_RS16020	3310547..3311284	-	738	WP_013098169.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12295.27 Da
• Isoelectric Point: 8.9951

>T164200 WP_044157892.1 NZ_CP056776:c3307025-3306699 [Enterobacter cloacae]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDPPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Nucleotide

• Length: 327 bp

>T164200 NZ_CP072826:4832203-4832586 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11917.60 Da
• Isoelectric Point: 7.0264

>AT164200 WP_007869729.1 NZ_CP056776:c3307363-3307046 [Enterobacter cloacae]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT164200 NZ_CP072826:4831961-4832203 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0M7FI99