Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1356193..1356950 Replicon chromosome
Accession NZ_CP056776
Organism Enterobacter cloacae strain DSM 30054

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag HWQ16_RS06620 Protein ID WP_044159004.1
Coordinates 1356193..1356675 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag HWQ16_RS06625 Protein ID WP_007372584.1
Coordinates 1356666..1356950 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ16_RS06600 1351253..1352533 - 1281 WP_176682058.1 type 4b pilus protein PilO2 -
HWQ16_RS06605 1352536..1354209 - 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
HWQ16_RS06610 1354229..1354672 - 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
HWQ16_RS06615 1354674..1355960 - 1287 WP_013095156.1 TcpQ domain-containing protein -
HWQ16_RS06620 1356193..1356675 - 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
HWQ16_RS06625 1356666..1356950 - 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
HWQ16_RS06630 1357106..1357606 - 501 WP_007372583.1 single-stranded DNA-binding protein -
HWQ16_RS06635 1357682..1358173 - 492 WP_007372582.1 DUF3577 domain-containing protein -
HWQ16_RS06640 1358806..1360818 - 2013 WP_044159002.1 DNA topoisomerase III -
HWQ16_RS06645 1360836..1361558 - 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1260111..1377757 117646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T164194 WP_044159004.1 NZ_CP056776:c1356675-1356193 [Enterobacter cloacae]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T164194 NZ_CP072826:c1583312-1582980 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT164194 WP_007372584.1 NZ_CP056776:c1356950-1356666 [Enterobacter cloacae]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT164194 NZ_CP072826:c1583570-1583313 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References