Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1059438..1060232 Replicon chromosome
Accession NZ_CP056776
Organism Enterobacter cloacae strain DSM 30054

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag HWQ16_RS05140 Protein ID WP_013095427.1
Coordinates 1059711..1060232 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag HWQ16_RS05135 Protein ID WP_013095428.1
Coordinates 1059438..1059707 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ16_RS05105 1055038..1056114 - 1077 WP_013095434.1 DUF2955 domain-containing protein -
HWQ16_RS05110 1056104..1057171 - 1068 WP_095440265.1 HlyD family secretion protein -
HWQ16_RS05115 1057168..1057638 - 471 WP_013095432.1 MarR family transcriptional regulator -
HWQ16_RS05120 1057842..1058537 - 696 WP_013095431.1 winged helix-turn-helix domain-containing protein -
HWQ16_RS05125 1058509..1058907 - 399 WP_013095430.1 amino acid-binding protein -
HWQ16_RS05130 1058900..1059223 - 324 WP_013095429.1 antibiotic biosynthesis monooxygenase -
HWQ16_RS05135 1059438..1059707 + 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
HWQ16_RS05140 1059711..1060232 + 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
HWQ16_RS05145 1060282..1061697 - 1416 WP_013095426.1 aldehyde dehydrogenase family protein -
HWQ16_RS05150 1061798..1062700 + 903 WP_013095425.1 LysR family transcriptional regulator -
HWQ16_RS05155 1062687..1063166 - 480 WP_013095424.1 carboxymuconolactone decarboxylase family protein -
HWQ16_RS05160 1063269..1064657 + 1389 WP_013095423.1 PLP-dependent aminotransferase family protein -
HWQ16_RS05165 1064744..1064905 - 162 WP_013095422.1 DUF1127 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T164192 WP_013095427.1 NZ_CP056776:1059711-1060232 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T164192 NZ_CP072826:c52141-50819 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCTGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTTACCGCCTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCACTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT164192 WP_013095428.1 NZ_CP056776:1059438-1059707 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT164192 NZ_CP072826:c52407-52141 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References