Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 518111..518731 Replicon chromosome
Accession NZ_CP056776
Organism Enterobacter cloacae strain DSM 30054

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag HWQ16_RS02655 Protein ID WP_013095889.1
Coordinates 518513..518731 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag HWQ16_RS02650 Protein ID WP_008499288.1
Coordinates 518111..518485 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ16_RS02640 513236..514429 + 1194 WP_013095891.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HWQ16_RS02645 514452..517598 + 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit -
HWQ16_RS02650 518111..518485 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
HWQ16_RS02655 518513..518731 + 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
HWQ16_RS02660 518941..519492 + 552 WP_013095888.1 maltose O-acetyltransferase -
HWQ16_RS02665 519608..520075 + 468 WP_013095887.1 membrane protein -
HWQ16_RS02670 520047..521507 - 1461 WP_013095886.1 PLP-dependent aminotransferase family protein -
HWQ16_RS02675 521609..522319 + 711 WP_013095885.1 GNAT family N-acetyltransferase -
HWQ16_RS02680 522316..522456 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HWQ16_RS02685 522459..522719 - 261 WP_006176933.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T164191 WP_013095889.1 NZ_CP056776:518513-518731 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T164191 NZ_CP072825:5472765-5473235 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT164191 WP_008499288.1 NZ_CP056776:518111-518485 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT164191 NZ_CP072825:5472325-5472768 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References