Detailed information of TA system TA164187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 3998941..4000636
• Replicon: chromosome
• Accession: NZ_CP056774
• Organism: Pseudomonas aeruginosa strain CDN129

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HWH78_RS18600
• Protein ID: WP_048910032.1
• Coordinates: 3999767..4000636 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: HWH78_RS18595
• Protein ID: WP_003112902.1
• Coordinates: 3998941..3999774 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWH78_RS18575	3994172..3995656	-	1485	WP_003163202.1	aldehyde dehydrogenase	-
HWH78_RS18580	3995668..3996471	-	804	WP_003112905.1	aspartate dehydrogenase	-
HWH78_RS18585	3996482..3998164	-	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
HWH78_RS18590	3998157..3998954	-	798	WP_003112903.1	SDR family oxidoreductase	-
HWH78_RS18595	3998941..3999774	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
HWH78_RS18600	3999767..4000636	-	870	WP_048910032.1	alpha/beta hydrolase	Toxin
HWH78_RS18605	4000645..4001175	-	531	WP_003162721.1	cupin domain-containing protein	-
HWH78_RS18610	4001189..4001950	-	762	WP_079274766.1	SDR family oxidoreductase	-
HWH78_RS18615	4001956..4002732	-	777	WP_003112898.1	ABC transporter permease	-
HWH78_RS18620	4002729..4003736	-	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
HWH78_RS18625	4003777..4004628	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30336.60 Da
• Isoelectric Point: 6.5894

>T164187 WP_048910032.1 NZ_CP056774:c4000636-3999767 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T164187 NZ_CP072825:c4886017-4885733 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCAGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT164187 WP_003112902.1 NZ_CP056774:c3999774-3998941 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT164187 NZ_CP072825:c4886249-4886007 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: Q9HYA1