Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1749298..1750095 Replicon chromosome
Accession NZ_CP056756
Organism Enterobacter hormaechei strain RHBSTW-00198

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag HV117_RS08390 Protein ID WP_032670168.1
Coordinates 1749574..1750095 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag HV117_RS08385 Protein ID WP_001303459.1
Coordinates 1749298..1749567 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV117_RS08355 1744838..1745197 + 360 WP_040117469.1 helix-turn-helix transcriptional regulator -
HV117_RS08360 1745523..1746764 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
HV117_RS08365 1746797..1747024 - 228 WP_006809324.1 YccJ family protein -
HV117_RS08370 1747043..1747639 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
HV117_RS08375 1748031..1748201 + 171 WP_001273664.1 general stress protein -
HV117_RS08380 1748318..1749223 + 906 WP_047051684.1 DMT family transporter -
HV117_RS08385 1749298..1749567 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
HV117_RS08390 1749574..1750095 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
HV117_RS08395 1750154..1751476 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
HV117_RS08400 1751498..1751989 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
HV117_RS08405 1752002..1752592 - 591 WP_047052687.1 malonic semialdehyde reductase -
HV117_RS08410 1752602..1753402 - 801 WP_047052686.1 pyrimidine utilization protein D -
HV117_RS08415 1753410..1753796 - 387 WP_032670161.1 pyrimidine utilization protein C -
HV117_RS08420 1753808..1754497 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T164152 WP_032670168.1 NZ_CP056756:1749574-1750095 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T164152 NZ_CP072802:302093-302196 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT164152 WP_001303459.1 NZ_CP056756:1749298-1749567 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT164152 NZ_CP072802:c302234-301963 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References