Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2993225..2994022 Replicon chromosome
Accession NZ_CP056751
Organism Enterobacter hormaechei strain RHBSTW-00216

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag HV128_RS14600 Protein ID WP_032670168.1
Coordinates 2993225..2993746 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag HV128_RS14605 Protein ID WP_001303459.1
Coordinates 2993753..2994022 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV128_RS14570 2988823..2989512 + 690 WP_047052685.1 pyrimidine utilization protein B -
HV128_RS14575 2989524..2989910 + 387 WP_032670161.1 pyrimidine utilization protein C -
HV128_RS14580 2989918..2990718 + 801 WP_047052686.1 pyrimidine utilization protein D -
HV128_RS14585 2990728..2991318 + 591 WP_047052687.1 malonic semialdehyde reductase -
HV128_RS14590 2991331..2991822 + 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
HV128_RS14595 2991844..2993166 + 1323 WP_048982825.1 pyrimidine utilization transport protein G -
HV128_RS14600 2993225..2993746 - 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
HV128_RS14605 2993753..2994022 - 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
HV128_RS14610 2994097..2995002 - 906 WP_047051684.1 DMT family transporter -
HV128_RS14615 2995119..2995289 - 171 WP_001273664.1 general stress protein -
HV128_RS14620 2995681..2996277 + 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
HV128_RS14625 2996296..2996523 + 228 WP_006809324.1 YccJ family protein -
HV128_RS14630 2996556..2997797 - 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
HV128_RS14635 2998123..2998482 - 360 WP_040117469.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T164137 WP_032670168.1 NZ_CP056751:c2993746-2993225 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T164137 NZ_CP072800:2269376-2269672 [Candidatus Thiothrix anitrata]
ATGCCACAATTAATTATTTCTGATAAAGCGCGGGCAGATATAGTCCGGTTTCATGCCTTTCTGAAACAACAAGATGTGAC
GGCTGCGAATAATGCGGTCTTTACCCTGCTGAATGCTTTTGGTGTCTTGGGGGGGCAGCCACACATCGGGCGGTTGGTGG
AGGATGAACCTGATTTGCGGGAGTTTTTGGTGGAATTTGGTAATAGTGGTTATGTTGCACTGTATCGTTATGACTCTAAA
CGAGATGCTGTGGTGGTGTTGACTATTCGCCATCAACGTGAAGTGGGTTATCAATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT164137 WP_001303459.1 NZ_CP056751:c2994022-2993753 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT164137 NZ_CP072800:2269122-2269388 [Candidatus Thiothrix anitrata]
ATGAGCGTCAAGCTTGACCTTAGTGACAGGGAACGCCTTGCATCTTTAGCGGCTACCCGCAACCGCACTTCCCATTTTTT
GATGAAACAGGCAGTGCAGGACTTCTTAGCGCGGGAAGAAGCCCGTCAAGCCTTTATTGCAGATGCTAAAGCCTCTTGGG
AGCATTACACCCAAACGGGGTTGCACGTTACCTTAGGGGAGTTGCGCCAGTGGTCAGATACACTTACGGCTAACCCTGAT
GCGCCGATGCCTGTATGCCACAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References